Cloning Vectors

Cloning Vectors Cloning vectors act as vehicles that can accept recombinant DNA for reproducing a large number of copies and insertion into new cells. The use of cloning vectors in molecular biology enables the editing, transfer, storage, and study of virtually any gene of interest. There are many types of vector backbones that can be used in cloning, such as yeast, adeno-associated viruses (AAV), plasmids, bacteriophages, cosmids, bacterial artificial chromosomes (BACs), and yeast artificial chromosomes (YACs). Vector choice can be determined by a number of factors, including the expression system, size of the DNA insert, selectable markers, restriction sites, as well as the desired copy number. Some cloning vectors may act as expression vectors as well, containing promoter regions and ribosome binding sites. When choosing, ensure that the gene of interest, cloning vector, downstream expression system are all compatible. Visit the supplier to see more details, such as vector maps and sequences.

Cloning Vectors

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Monster Green Fluorescent Protein phMGFP Vector
  • Quantity:
    20microg
  • Description:
    The Monster Green Fluorescent Protein encoded by the hMGFP gene is an ideal fluorescent reporter, providing high-level fluorescence and reducing ...
  • Vector Type:
    Plasmid
Citations:
(12)
pAdVAntage Vector
  • Quantity:
    20microg
  • Description:
    Co-transfection of mammalian cells with the pAdVAntage Vector enhances transient protein expression in a variety of cell types by increasing ...
  • Vector Type:
    Adenoviral
Citations:
(70)
pCMVTNT Vector

pCMVTNT Vector

Promega

  • Quantity:
    20microg
  • Description:
    The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from ...
  • Vector Type:
    Plasmid
Citations:
(7)
pGEM-3Z Vector

pGEM-3Z Vector

Promega

  • Quantity:
    20microg
  • Description:
    Intended for use as a standard cloning vector or for high-fidelity efficient synthesis of RNA in vitro
  • Vector Type:
    Plasmid
Citations:
(9)
pGEM-3Zf(+) Vector
  • Quantity:
    20microg
  • Description:
    Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA
  • Vector Type:
    Plasmid
Citations:
(6)
pGEM-3Zf(-) Vector
  • Quantity:
    20microg
  • Description:
    Used as a standard cloning vector, a template for in vitro transcription or for production of circular ssDNA
  • Vector Type:
    Plasmid
Citations:
(3)
pGEM-4Z Vector

pGEM-4Z Vector

Promega

  • Quantity:
    20microg
  • Description:
    Intended for use as a standard cloning vector or for the high-fidelity efficient synthesis of RNA in vitro
  • Vector Type:
    Plasmid
Citations:
(8)
pGEM-5Zf(+) Vector
  • Quantity:
    20microg
  • Description:
    Used as a standard cloning vector and as a template for in vitro transcription
  • Vector Type:
    Plasmid
pGEM-7Zf(+) Vector
  • Quantity:
    20microg
  • Description:
    Used as a standard cloning vector, template for in vitro transcription or for production of circular ssDNA
  • Vector Type:
    Plasmid
Citations:
(4)
pRL-CMV Vector

pRL-CMV Vector

Promega

  • Quantity:
    20microg
  • Description:
    The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used ...
  • Vector Type:
    Plasmid
Citations:
(189)
pRL-SV40 Vector

pRL-SV40 Vector

Promega

  • Quantity:
    20microg
  • Description:
    The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used ...
  • Vector Type:
    Plasmid
Citations:
(323)
Figures:
(1)
pRL-TK Vector

pRL-TK Vector

Promega

  • Quantity:
    20microg
  • Description:
    The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used ...
  • Vector Type:
    Plasmid
Citations:
(553)
Figures:
(1)
pRL-null Vector

pRL-null Vector

Promega

  • Quantity:
    20microg
  • Description:
    The pRL Vectors are wildtype Renilla luciferase control reporter vectors, which provide constitutive expression of Renilla luciferase and can be used ...
  • Vector Type:
    Plasmid
Citations:
(21)
pSP72 Vector

pSP72 Vector

Promega

  • Quantity:
    20microg
  • Description:
    Used as standard cloning vector or for transcription of RNA in vitro
  • Vector Type:
    Plasmid
Citations:
(13)
pSP73 Vector

pSP73 Vector

Promega

  • Quantity:
    20microg
  • Description:
    Used as standard cloning vector or for transcription of RNA in vitro
  • Vector Type:
    Plasmid
Citations:
(13)
pTNT Vector

pTNT Vector

Promega

  • Quantity:
    20microg
  • Description:
    The pCMVTNT and pTNT Vectors are designed for convenient expression of cloned genes in vitro or in vivo. SP6 and T7 promoters allow expression from ...
  • Vector Type:
    Plasmid
Citations:
(18)
CheckMate Negative Control Vectors
  • Quantity:
    1 set
  • Description:
    The pACT-MyoD and pBIND-Id Control Vectors can be used together as negative controls when co-transfected with the reporter vector pGL4.31[luc2P/...
  • Vector Type:
    Plasmid
CheckMate Positive Control Vectors
  • Quantity:
    1 set
  • Description:
    The pACT-MyoD and pBIND-Id Control Vectors can be used together as positive controls when co-transfected with the reporter vector pGL4.31[luc2P/...
  • Vector Type:
    Plasmid
HaloTag Control Vector
  • Quantity:
    20microg
  • Description:
    The HaloTag Mammalian Pull-Down Systems capture and purify intracellular protein complexes for correlative cellular localization and real-time imaging...
  • Vector Type:
    Plasmid
Citations:
(15)
HaloTag Flexi Vectors-CMV Deletion Series Sample Pack
  • Quantity:
    9 x 2microg
  • Description:
    Vectors in the sample pack are configured to append the HaloTag protein to either the carboxy- (pFC vectors) or amino-terminus (pFN vectors) of the ...
  • Vector Type:
    Plasmid
Citations:
(2)
pBIND-ER(alpha) Vector
  • Quantity:
    20microg
  • Description:
    NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR ...
  • Vector Type:
    Plasmid
Citations:
(1)
pBIND-GR Vector

pBIND-GR Vector

Promega

  • Quantity:
    20microg
  • Description:
    NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR ...
  • Vector Type:
    Plasmid
Citations:
(5)
pBR322 Vector

pBR322 Vector

Promega

  • Quantity:
    10microg
  • Description:
    pBR322 Vector
  • Vector Type:
    Plasmid
Citations:
(1)
pCI Mammalian Expression Vector
  • Quantity:
    20microg
  • Description:
    The pCI Vector contains the CMV immediate-early enhancer/promoter region and is designed to promote constitutive expression of cloned DNA inserts in ...
  • Vector Type:
    Plasmid
Citations:
(37)
pCI-neo Mammalian Expression Vector
  • Quantity:
    20microg
  • Description:
    Contains a neomycin resistance gene and the CMV immediate-early enhancer/promoter region to promote constitutive expression of cloned DNA inserts in ...
  • Vector Type:
    Plasmid
Citations:
(58)
pF1A T7 Flexi Vector
  • Quantity:
    20microg
  • Description:
    These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied ...
  • Vector Type:
    Plasmid
pF1K T7 Flexi Vector
  • Quantity:
    20microg
  • Description:
    These untagged vectors are used for inducible expression of proteins in E. coli and cell-free systems using the T7 RNA polymerase promoter. Supplied ...
  • Vector Type:
    Plasmid
pF25A ICE T7 Flexi Vector
  • Quantity:
    20microg
  • Description:
    Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region ...
  • Vector Type:
    Plasmid
Citations:
(2)
pF25K ICE T7 Flexi Vector
  • Quantity:
    20microg
  • Description:
    Designed for use with Insect Cell Extracts and contain untranslated region (UTR) sequences at the 5´ and 3´ ends of the gene coding region ...
  • Vector Type:
    Plasmid
pF3A WG (BYDV) Flexi Vector
  • Quantity:
    20microg
  • Description:
    Designed for use with Wheat Germ extracts and contain sequences from the barley yellow dwarf virus (BYDV), an RNA plant virus, upstream and downstream...
  • Vector Type:
    Plasmid
Citations:
(4)
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Promega
E1310 | 20microg
1
1 Figure from 15 Publications
Promega
E1171 | 20microg
4
1 Figure from 25 Publications
Promega
N1021 | 20microg
Extended Data  10
1 Figure from 9 Publications
Promega
E2231 | 20microg
3
1 Figure from 323 Publications
Promega
E2241 | 20microg
2
1 Figure from 553 Publications
Promega
E1081 | 20microg
1
1 Figure from 48 Publications
Showing 6 of 6 products
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