pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) TNFα-dependent modulation of WT1-MMP9 regulatory axis links developmental and inflammatory pathways in glaucomaiScienceSeptember 18, 2026Tahseen Ahmed, Jyotishman Sarma, Meha Kabra, Shantanu Saha Roy, Samir Bera, Arun Kumar Mishra, Diganta Roy, Sayani Bagchi, Goutham Pyatla, Ashish Mishra, et al.expression plasmid,OriGene,Cat #RC220079 pGL3-Basic vector,Promega,212936 pRL-TK Renilla plasmid,Promega,E2241 ,, Oligonucleotides,Oligonucleotides,Oligonucleotides ,, wt1 morpholino,Gene Tools,Sequence in Table

(2) Demethylase FTO attenuates atherosclerotic plaque via upregulating ABCA1 and ABCG1 expression by targeting ATG5Mol Med RepSeptember 16, 2026Fengxia Guo, Mei He, Yaonan Li, Kangning Zhu, Bing Hufirefly luciferase reporter, and the pRL-TK plasmid, serving as the Renilla luciferase control vector (E2241; Promega Corporation). Additionally, shRNAs targeting either FTO (5′-GGACTTAAGGAATCCAGAATT-3′) or YTHDF1

(3) Honokiol Suppresses Stemness and Sensitizes Triple-Negative Breast Cancer to Chemotherapy via YAP/TAZ-TEAD InhibitionOncology ResearchSeptember 14, 2026Jiang-Nan Xia, Shan-Dong Zhu, Wei-Ling Qu, Yi-Lin Hu, Wen-Yi Ma, Wenyan Wang, Qian-Lan Huang, Bing-Yuan Lin, Jia-En Guo, Ying-Wei Licontains a synthetic promoter responsive to YAP/TAZ, and 10 ng of the pRL-TK Renilla vector (Promega, #E2241, Madison, WI, USA). After a 24-h recovery period, the transfected cells were treated with honokiol (10,

(4) Liposomal Doxorubicin Induces PD-L1-High Tumor-Associated Macrophages and Sensitizes Triple-Negative Breast Cancer to PD-L1 BlockadeOncol ResSeptember 14, 2026Yu Zheng, Peng Zheng, Yidan Zheng, Zihan Xi, Tao HuangBiotechnology Co., Ltd. (Beijing, China). The pRL-TK Renilla luciferase control plasmid (Promega Corporation, E2241, Madison, WI, USA) was used as an internal control. BMDMs cultured in 24-well plates were co-transfected

(5) DNA Polymerase θ Drives Colorectal Cancer Progression through Wnt/β-Catenin Activation and Shows Potential Association with Immunosuppressive Microenvironment RemodelingOncology ResearchSeptember 14, 2026Li Li, Jianhua Wang, Feilong Zhou, Lingjun Geng, Kongwang HuUSA). The pRL-TK Renilla luciferase internal control plasmid was purchased from Promega Corporation (E2241, Madison, WI, USA). Stable POLQ-knockdown (shRNA) and stable POLQ-overexpressing SW480 and HCT116 cell

(6) Targeting LOXL4-driven matrix stiffening with acetyldigoxin restores CD8+ T cell function in lung cancerSci AdvSeptember 11, 2026Xuyu Gu, Yifei Zhu, Li Xu, Xinnan Xu, Kaiqi Jin, William C. Cho, Qiyu Fangreporter, 250 ng of YAP1 or YBX1 expression plasmid, and 50 ng of Renilla control (pRL-TK; Promega, #E2241) using Lipofectamine 3000. Control groups received equivalent empty vector (pcDNA3.1). Transfection

(7) NAT10 is critical to block RNA sensing-induced IFN-β transactivation in viral infectionmBioSeptember 9, 2026Youngmin Park, Yan Liu, Jinshan He, Suha Eleya, Zhenyu Wu, Guillaume Nicolas Fiches, Dawei Zhou, Emily G. Watters, Zhixian M. He, Kateepe Arachchige Sudeera Nuwan Shanaka, et al.provided by Dr. Jacob S. Yount, Ohio State University) and pRL-TK renilla luciferase vector (Cat. #E2241, Promega) along with pTRIP-SFFV-mtagBFP-2A RIGI WT (Cat. #167289, Addgene) or pEBG-RIG-I 2CARD-GST (kindly

(8) A Truncated Serpin D1 Variant Regulates Adseverin-Dependent Actin Severing and Is Required for OsteoclastogenesisCellsSeptember 3, 2026Yongqiang Wang, Chenfan Ji, Andrew Y. Wang, Masoud Norouzi, Isabel Ding, Michael Glogauer, Mark Abovsky, Igor Jurisica, Christopher A. McCulloch(passage 6, 1.5 × 106) with 150 ng Renilla internal control plasmid construct pRL-TK (Promega, Cat. No. E2241 (20:1), by using an Amaxa nucleofector (Walkersville, MD, USA, program D-032) with buffer 1M reagent

(9) Novel TBX20 Variations Susceptible to Sporadic Atrial FibrillationBiomedicinesSeptember 3, 2026Zhen-Yu Xu, Dao-Liang Zhang, Xing-Biao Qiu, Chen-Xi Yang, Ying-Jia Xu, Yi-Qing Yang, Ning Liof the KCNH2-luc plasmid and 0.04 μg of the pRL-TK plasmid expressing renilla luciferase (Cat. No. E2241; Promega, Madison, WI, USA). As described previously [83,88], the HeLa and COS7 cells were collected

(10) Mechanism of miR-188-5p enhancing radioresistance in nasopharyngeal carcinoma by targeting PTEN to activate the PI3K/Akt signaling pathwayTranslational Cancer ResearchAugust 31, 2026Fuming Zhou, Jia Wan, Haiyan Zhang, Feng Chen, Jin Wang, Yanli Yang, Huan WangpGL3-luc-PTEN UTR WT and MUT reporter plasmids. The pRL-TK Renilla luciferase control vector (Promega, E2241) was used for normalisation of transfection efficiency. Cells were seeded in 96-well plates at a density

(11) FOXO6 inhibits SMURF2-mediated ubiquitination and degradation of NR4A1 to promote retinal pigment epithelial cell ferroptosis in diabetic retinopathyJ Cell Commun SignalAugust 26, 2026Qiao Chen, Bo Su, Ke Xu, Liang Li, Changzheng Chenco‐transfected with 0.8 μg of pGL3‐WT or pGL3‐Mut, 0.02 μg of pRL‐TK Renilla luciferase plasmid (Cat# E2241, Promega) as an internal control, and either sh‐NC or sh‐FOXO6 plasmids using Lipofectamine 3000. After

(12) CUDC-907 attenuates pulmonary fibrosis by reversing HDAC1-mediated SMAD4 deacetylation at lysine 45Biology DirectAugust 25, 2026Yu Li, Yuan Cui, Haoyu Qiu, Kai Fu, Chenye Shao, Zhike Chen, Jian Yang, Jiawei Chen, Xin Lv, Xiumin Zhou, et al.SBE-luc reporter plasmid (Addgene #16495) and 50 ng of pRL-TK Renilla luciferase plasmid (Promega #E2241) per well using Lipofectamine 3000 transfection reagent according to the manufacturer’s instructions.

(13) Dysregulation of the KLF9/TXNRD2 Axis Leads to Mitochondrial DNA Oxidation and NLRP3 Inflammasome Activation in Ulcerative ColitisJ Biochem Mol ToxicolAugust 24, 2026Yijia Fan, Lingling Dai, Feng Zhu, Mingfang Ping, Xiaofeng Zhu, Junguo Chenconfluence, co‐transfected with 200 ng of pGL3‐TXNRD2 promoter reporter, 50 ng of pRL‐TK internal control (E2241, Promega), and 200 ng of thKLF9 overexpression vector (pcDNA3.1‐KLF9) or an empty vector (pcDNA3.1)

(14) NAP1 switches from an activator to a limiter of interferon induction by trapping TBK1 in condensatesNature CommunicationsAugust 19, 2026Damien Glon, Quentin Riller, Lucía Álvarez, Louise Gioja, Frédéric Rivière, Benjamin Léonardon, Zackie Aktary, Ariane Guillemot, Laïla Sago, Olivier Pellé, et al.pcDNA3.1_IRF3-5D were previously described31,58,59. The plasmid pRL-TK was purchased from Promega Corporation (#E2241). The plasmids psPAX2 (Addgene, 12260) and pMD2.G (Addgene, 12259) come from Didier Trono’s lab (EPFL,

(15) FHL3 Regulates Vascular Smooth Muscle Cell Phenotypic Switching Through the MRTFB-SRF Signaling AxisCellsAugust 17, 2026Xiaoxin Huang, Heming Zhang, Yanhong Zhang, Charles U. Solomon, David G. McVey, Shu Yepreviously described [29]. Each of these constructs, together with the pRL-TK control plasmid (Promega, E2241), were transfected into HASMCs, and luciferase activity was measured 2 days post-transfection using

(16) SOX4 facilitates osteogenic differentiation through suppressing NLRP3 transcription-mediated pyroptosisCytotechnologyAugust 8, 2026Ping Li, Xue-Yi Lin, Yu Lin, Yan Liu, Wei-Bing YangpGL3-mut-P2 plasmid, 0.2 µg of pcDNA3.1-SOX4 or empty vector, and 0.05 µg of pRL-TK vector (Promega, Cat. No. E2241, USA) as an internal control, using Lipofectamine 3000 reagent. After 48 h, luciferase activity was

(17) Loss of SETD2-mediated H3K36me3 Drives CD8+ T Cell Exhaustion in Lung Cancer through CXCL-dependent PMN-MDSC Recruitment and Arginine DeprivationResearch (Wash D C)August 5, 2026Xuyu Gu, Junjie Zhu, Tao Ge, Keyi Chen, Yang Yang, Kaiqi Jin, Xinnan Xuconfluence). Cells were cotransfected with 1 μg of promoter–reporter plasmid and 50 ng of pRL-TK (#E2241, Promega) as internal control. Medium was replaced 6 h later, followed by another 24 h. Cells were lysed

(18) Loss of NCCRP1 overcomes immune evasion in lung adenocarcinomaJ Immunother CancerAugust 5, 2026Lu Liu, Shihao Qi, Liye Shao, Junyang Cai, Qingqing Zeng, Xu Jiang, Fang Ma, Weiwei Lin(China). SgCtrl or sgNccrp1 LLC cells were co-transfected with each reporter plasmid and the pRL-TK (E2241, Promega) plasmid using the liposomal transfection reagent GTR. After 48 hours of transfection, firefly

(19) TMEM135 deficiency prevents intestinal lipid accumulation by coordinating lipid uptake and oxidation through ELOVL6-mediated PPARα activationExperimental & Molecular MedicineAugust 5, 2026Hyunsoo Kim, Channy Park, Xiaofan Wei, Arun Chhetri, Laxman Manandhar, Jiwon Seo, Yoonkyung Park, Sang-Wook Lee, Raekil ParkPPRE-driven firefly luciferase reporter plasmid together with the Renilla luciferase control vector pRL-TK (E2241, Promega, USA) using Lipofectamine 3000 (Thermo Fisher Scientific), according to the manufacturer’s

(20) Transcription Factor MAX Regulates Liver Cancer Cell Growth, Migration, Invasion, and Epithelial–Mesenchymal Transition by Promoting SF3A3 ExpressionWorld Journal of OncologyAugust 1, 2026Gui Zhou, Mei Yu Dai, Xiang Chen, Li Ming Liu, Fa Quan Linhairpin RNA (shRNA) targeting MAX (100 ng), and the Renilla luciferase control vector pRL-TK (10 ng, Cat# E2241, Promega) using Lipofectamine 3000 (Thermo Fisher). After 48 h, the cells were lysed and luciferase

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pRL-TK Vector

pRL-TK Vector from Promega

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Specifications/Features

Promega

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pRL-TK Vector from Promega

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