pGEM-4Z Vector from Promega

Product Specs
ItempGEM-4Z Vector
CompanyPromega
Price $66.00Supplier Page View Company Product Page
Catalog NumberP2161
HostBacteria
PromoterSP6 and T7 RNA polymerase promoters
MCSEcoR I, Sac I, Kpn I, Ava I, Xma I, Sma I, BamH I, Xba I, Sal I, Acc I, Hinc II, Pst I, Sph I, Hind III
ResistanceAmpR
Selectable MarkerBlue/white screening for recombinants
Origin of ReplicationpUC18
Quantity20microg
Vector NamepGEM^®-4Z

Specifications/Features

Features:
• Blue/White Screening: Allows the easy identification of recombinant clones.
• Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
• Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

Vector Size:
2746 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Clusterin activates the heat shock response via the PI3K/Akt pathway to protect cardiomyocytes from high-temperature-induced apoptosisOpen Life SciencesMarch 28, 2025Jianguo Zhou, Xiupan Lu, Yiming Xie, Guangyao MaoLY294002 (10 μM) [19]. For cell transfection, the CDS sequence of CLU was cloned into the pGEM®-4Z Vector (P2161, Promega, Wisconsin, USA) to construct the CLU overexpression vector (oe-CLU). When cells reached 70–90%

(2) Expanded specific T cells to hypomutated regions of the SARS-CoV-2 using mRNA electroporated antigen-presenting cellsMolecular Therapy - Methods & Clinical DevelopmentMarch 14, 2024Elizabeth Ogando-Rivas, Paul Castillo, Changlin Yang, Vrunda Trivedi, Dingpeng Zhang, Fernanda Pohl-Guimarães, Ruixuan Liu, Arnav Barpujari, Kate M. Candelario, Hector Mendez-Gomez, et al.respectively). Subsequently, Spike CDS fragment was cloned into a modified pGEM4z (called pGEM4zR, Promega, P2161) plasmid that contains a T7 promoter and a 62 nucleotide long poly A sequence, creating pGEM4zR-Spike

(3) Hepatitis B virus X protein promotes liver cell pyroptosis under oxidative stress through NLRP3 inflammasome activationInflammation research : official journal of the European Histamine Research Society ... [et al.]July 1, 2020Xie WH, Ding J, Xie XX, Yang XH, Wu XF, Chen ZX, Guo QL, Gao WY, Wang XZ, Li D.pcDNA3.1 (pNC) was maintained in our laboratory. The pGEM-4Z (pGEM) plasmid was purchased from Promega (#P2161; Madison, WI, USA). The pHBV plasmid expressing full-length wild-type HBV genomic complementary DNA

(4) Dynamic PML protein nucleolar associations with persistent DNA damage lesions in response to nucleolar stress and senescence-inducing stimuliAging (Albany NY)September 7, 2019Terezie Imrichova, Sona Hubackova, Alena Kucerova, Jan Kosla, Jiri Bartek, Zdenek Hodny, Pavla Vasicovawas prepared as follows: PCR-amplified PML IV cDNA was inserted into HincII-digested pGEM4z plasmid (P2161, Promega, Madison, WI, USA) and PML IV was subsequently excised and inserted into pEGFP-C3 plasmid (6082-1,

(5) Identification of RBPMS as a mammalian smooth muscle master splicing regulator via proximity of its gene with super-enhancerseLifeJuly 8, 2019Erick E Nakagaki-Silva, Clare Gooding, Miriam Llorian, Aishwarya G Jacob, Frederick Richards, Adrian Buckroyd, Sanjay Sinha, Christopher W J Smith22-RBPMSA,This study,pInducer22-RBPMSA,See Materialsand methods Recombinant DNA reagent,pGEM4Z,Promega,Promega: P2161,in vitrotranscription templates; see Material and methods Sequence-based reagent,oligonucleotides,Sigma Aldrich

(6) Maintenance of Cardiolipin and Crista Structure Requires Cooperative Functions of Mitochondrial Dynamics and Phospholipid TransportCell ReportsJanuary 15, 2019Rieko Kojima, Yuriko Kakimoto, Shiina Furuta, Kie Itoh, Hiromi Sesaki, Toshiya Endo, Yasushi TamuraDNA pBS-kanMX4,Endo Lab,N/A pBS-hphMX,Endo Lab,N/A pBS-CgHIS3,Endo Lab,N/A pGEM-4Z,Promega,Cat#: P2161 pGEM-4Z-Crd1,This study,N/A pGEM-4Z-Psd1,This study,N/A pGEM-4Z-Hsp60,"Tamura et al., 2009",N/A pGEM-4Z-Tim23,"Tamura

(7) Major Histocompatibility Complex Class I Allele-specific Cooperative and Competitive Interactions between Immune Evasion Proteins of CytomegalovirusJournal of Experimental MedicineSeptember 16, 2002Markus Wagner, Anja Gutermann, Jürgen Podlech, Matthias J. Reddehase, Ulrich H. Koszinowskiamplificate was then ligated into cloning vector pGEM-4Z (GenBank/EMBL/DDBJ accession no. X65305) (no. P2161; Promega). Probe m152-P was tagged during the PCR by incorporation of digoxigenin-11-dUTP (no. 1093088;

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pGEM-4Z Vector

pGEM-4Z Vector from Promega

Description

Specifications/Features

Promega

Citations (7)

Reviews

pGEM-4Z Vector from Promega

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