pGloSensor-20F cAMP Plasmid from Promega

Product Specs
ItempGloSensor-20F cAMP Plasmid
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1171
HostMammalian
PromoterCMV
Vector NamepGloSensor-20F
Quantity20microg
Selectable MarkerAmpicillin
Origin of ReplicationColE1-derived plasmid origin of replication
ResistanceAmpicillin
Molecular Weight~40kDa (CDK6); ~35kDa (CyclinD3)
TypeEnzyme

Specifications/Features

Vector Size:
6990 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Glucagon Receptor Deficiency Causes Early-Onset Hepatic SteatosisDiabetesOctober 1, 2025Tessa M. Cacciottolo, Katherine Lawler, Kevin M. Méndez-Acevedo, Edson Mendes de Oliveira, Adam Syanda, Elana Henning, Julia M. Keogh, Rebecca Bounds, Miriam Smith, Daniyal Ashraf, et al.with GCGR (WT or mutant, 20 ng/well) and pGloSensor-20F cAMP reporter plasmid (100 ng/well; Promega, E1171) using Lipofectamine 2000. After 24 h, medium was replaced with 90 µL of 2% v/v GloSensor cAMP reagent

(2) Understanding the genetic complexity of puberty timing across the allele frequency spectrumNature GeneticsJuly 1, 2024Katherine A. Kentistou, Lena R. Kaisinger, Stasa Stankovic, Marc Vaudel, Edson Mendes de Oliveira, Andrea Messina, Robin G. Walters, Xiaoxi Liu, Alexander S. Busch, Hannes Helgason, et al.After 24 h, cells were transfected with both 100 ng per well of pGloSensorTM-20F cAMP plasmid (Promega, E1171) and 30 ng per well of each plasmid encoding either MC3R or MC3R and GPR83, using Lipofectamine 2000

(3) The Discovery of Novel α2a Adrenergic Receptor Agonists Only Coupling to Gαi/O Proteins by Virtual ScreeningInternational Journal of Molecular SciencesJune 30, 2024Peilan Zhou, Fengfeng Lu, Huili Zhu, Beibei Shi, Xiaoxuan Wang, Shiyang Sun, Yulei Li, Ruibin Sumanufacturer protocols, HEK293 cells were co-transfected with the pGloSensor-22F cAMP plasmid (Promega, E1171) and pCMV6 Entry-Flag-α2A-AR. After 24 h, the cells were seeded in white 96-well plates. The next day,

(4) Systematic characterization of multi-omics landscape between gut microbial metabolites and GPCRome in Alzheimer’s diseaseCell ReportsMay 28, 2024Yunguang Qiu, Yuan Hou, Dhruv Gohel, Yadi Zhou, Jielin Xu, Marina Bykova, Yuxin Yang, James B. Leverenz, Andrew A. Pieper, Ruth Nussinov, et al.87792 Protease/Phosphatase Inhibitor,Thermo Scientific,Cat# PI78442 pGloSensor™-22F cAMP plasmid,Promega,E1171 GPR84 plasmid,Addgene,RRID: Addgene_66377 GloSensor™ cAMP Reagent,Promega,E1290 DMEM,Gibco,10313021 Fetal

(5) Stabilization of interdomain interactions in G protein α subunits as a determinant of Gα(i) subtype signaling specificityThe Journal of biological chemistryMay 1, 2024Lefevre TJ, Wei W, Mukhaleva E, Meda Venkata SP, Chandan NR, Abraham S, Li Y, Dessauer CW, Vaidehi N, Smrcka AV.were transfected with the following plasmids and amounts per well: 50 ng GloSensor −20F cAMP plasmid (E1171, Promega), 125 ng Gαi or Gαi QL in pcDNA3.1+. In Gαi titration experiments, DNA was supplemented with

(6) Low resting metabolic rate and increased hunger due to β-MSH and β-endorphin deletion in a canine modelSci AdvMarch 8, 2024Marie T. Dittmann, Gabriella Lakatos, Jodie F. Wainwright, Jacek Mokrosinski, Eloise Cross, I. Sadaf Farooqi, Natalie J. Wallis, Lewis G. Halsey, Rory Wilson, Stephen O'Rahilly, et al.cells were transfected with both -20F cAMP reporter plasmid (100 ng per well; Promega, catalog no. E1171) and of MCR plasmid [30 ng per well; human or canine MC3R or MC4R with pcDNA3.1(+) backbone was used

(7) Novel Scaffold Agonists of the α2A Adrenergic Receptor Identified via Ensemble-Based StrategyMoleculesFebruary 29, 2024Shiyang Sun, Pengyun Li, Jiaqi Wang, Dongsheng Zhao, Tingting Yang, Peilan Zhou, Ruibin Su, Zhibing Zheng, Song LiIn Vitro HEK293 cells were co-transfected with the pGloSensor-22F cAMP plasmid (Promega, Madison, WI, USA, E1171) and pCMV6 Entry-Flag-α2A-AR, following the procedure of GloSensor cAMP biosensor (Promega) manufacturer’s

(8) Understanding the genetic complexity of puberty timing across the allele frequency spectrummedRxivJuly 21, 2023Katherine A Kentistou, Lena R Kaisinger, Stasa Stankovic, Marc Vaudel, Edson M de Oliveira, Andrea Messina, Robin G Walters, Xiaoxi Liu, Alexander S Busch, Hannes Helgason, et al.After 24 hours, cells were transfected with both 100 ng/well of pGloSensorTM-20F cAMP plasmid (Promega, E1171) and 30 ng/well of each plasmid encoding either MC3R or MC3R and GPR83, using Lipofectamine 2000 (GIBCO,

(9) Stabilization of Interdomain Interactions in G protein α i Subunits Determines Gα i Subtype Signaling SpecificitybioRxivApril 12, 2023Tyler J. Lefevre, Wenyuan Wei, Elizaveta Mukhaleva, Sai Pranathi Meda Venkata, Naincy R. Chandan, Saji Abraham, Yong Li, Carmen W. Dessauer, Nagarajan Vaidehi, Alan V. Smrckawere transfected with the following plasmids and amounts per well: 50 ng GloSensor −20F cAMP plasmid (E1171, Promega), 125 ng Gαi or Gαi QL in pcDNA3.1+. In Gαi titration experiments, DNA was supplemented with

(10) S-nitrosylation is required for β(2)AR desensitization and experimental asthmaMolecular cellAugust 18, 2022Fonseca FV, Raffay TM, Xiao K, McLaughlin PJ, Qian Z, Grimmett ZW, Adachi N, Wang B, Hausladen A, Cobb BA, et al.study,N/A ,, Recombinant DNA,Recombinant DNA,Recombinant DNA GloSensor-20F cAMP biosensor,Promega,E1171 CAMYEL cAMP biosensor,"Paul Sternweis, UT Southwestern, Jiang et al., 2007 PMID 17283075",N/A Flag-β2AR

(11) The organic cation transporter 2 regulates dopamine D1 receptor signaling at the Golgi apparatuseLifeApril 25, 2022Puri NM, Romano GR, Lin TY, Mai QN, Irannejad R.R2014b,MathWorks,DOI:10.5281/zenodo.5146169,"Bakr et al., 2021" Recombinant DNA reagent,pGloSensor-20F,pGloSensor-20F,Promega,E1171, Recombinant DNA reagent,Signal sequence Snap-D1DR,Signal sequence Snap-D1DR,This study,"pCDNA3 backbone;

(12) A network of Gα(i) signaling partners is revealed by proximity labeling proteomics analysis and includes PDZ-RhoGEFScience signalingJanuary 18, 2022Chandan NR, Abraham S, SenGupta S, Parent CA, Smrcka AV.(655983, Greiner). The following amounts of DNA were used per well: 50 ng of pGloSensor™-20F cAMP Plasmid (E1171, Promega), 125 ng of untagged Gαi1-WT, Gαi1-QL or BioID2 fused Gαi clones or empty vector (control,

(13) Efficient optogenetic silencing of neurotransmitter release with a mosquito rhodopsinNeuronMay 11, 2021Mathias Mahn et al.commercial assays,Critical commercial assays,Critical commercial assays GloSensor cAMP Assay,Promega,Cat#E1171 Experimental models: Cell lines,Experimental models: Cell lines,Experimental models: Cell lines HEK293T,Sigma-Aldrich,Cat#12022001RRID:CVCL_0063 Experimental models

(14) Spatial decoding of endosomal cAMP signals by a metastable cytoplasmic PKA networkNature Chemical BiologyMay 1, 2021Grace E. Peng, Veronica Pessino, Bo Huang, Mark von ZastrowEMCV (IRES) Rluc (Boca Scientific, Dedham, MA; OG296) and from pGloSensor-20F (Promega, Madison, WI; E1171). The full length GloSensor20F coding sequence including stop codon was amplified by PCR and inserted

(15) Human MC4R variants affect endocytosis, trafficking and dimerization revealing multiple cellular mechanisms involved in weight regulationCell reportsMarch 23, 2021Brouwers B, de Oliveira EM, Marti-Solano M, Monteiro FBF, Laurin SA, Keogh JM, Henning E, Bounds R, Daly CA, Houston S, et al.After 24 hours, cells were transfected with both 100 ng/well of pGloSensor™-20F cAMP plasmid (Promega, E1171) and 30 ng/well of plasmid encoding either MC4R WT or MC4R mutants (MC4R-FLAG in pcDNA3.1(+)), using

(16) Human gain-of-function MC4R variants show signaling bias and protect against obesityYearbook of Paediatric EndocrinologyOctober 23, 2020Lotta LA et al.cells were then transfected with both 100 ng/well 703 of pGloSensorTM-20F cAMP plasmid (Promega, E1171) and 30 ng/well of plasmid encoding 704 either WT/mutant MC4R. The day after transfection, cell media

(17) A high-throughput CRISPR interference screen for dissecting functional regulators of GPCR/cAMP signalingPLoS geneticsOctober 1, 2020Semesta KM, Tian R, Kampmann M, von Zastrow M, Tsvetanova NG.luciferase into which a cAMP-binding domain has been inserted from the pGloSensor-20F (Promega, Cat #E1171). Cell culture and reporter cell line generation HEK293 and HEK293T cells were obtained from ATCC

(18) G protein-regulated endocytic trafficking of adenylyl cyclase type 9eLifeJune 9, 2020Lazar AM, Irannejad R, Baldwin TA, Sundaram AB, Gutkind JS, Inoue A, Dessauer CW, Von Zastrow M.(human),HA-G(alpha)s-Q227L,"Masters et al., 1989",,pcDNA3 backbone Recombinant DNA reagent,pGloSensor-20F,Promega,E1171, Transfected construct (human),AC9-siRNA,This study,,See Materials and methods for sequence Transfected

(19) Molecular evolution of the proopiomelanocortin system in Barn owl speciesPloS oneJanuary 1, 2020Löw K, Ducrest AL, San-Jose LM, Simon C, Uva V, Seidah NG, Pasquato A, Kunz S, Roulin A.Human MCR plasmid or IRES GFP control plasmid in combination with 100 ng pGloSensorTM-20F cAMP plasmid (E1171, Promega Inc., Madison, Wisconsin, USA). Medium was removed 24 hours after transfection and replaced

(20) Human Gain-of-Function MC4R Variants Show Signaling Bias and Protect against ObesityCellApril 18, 2019Lotta LA, Mokrosiński J, Mendes de Oliveira E, Li C, Sharp SJ, Luan J, Brouwers B, Ayinampudi V, Bowker N, Kerrison N, et al.24 hours, cells were then transfected with both 100 ng/well of pGloSensor-20F cAMP plasmid (Promega, E1171) and 30 ng/well of plasmid encoding either WT/mutant MC4R. The day after transfection, cell media were

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