pGL4.50[luc2/CMV/Hygro] Vector from Promega

Product Specs
ItempGL4.50[luc2/CMV/Hygro] Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1310
HostMammalian
Vector NamepGL4.50
Quantity20microg
PromoterMinimal promoter
Selectable MarkerHygromycin
ResistanceAmpicillin
Origin of ReplicationColE1-derived plasmid origin of replication

Specifications/Features

Vector Size:
6600 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Targeting GRPR for sex hormone-dependent cancer after loss of E-cadherinNatureJune 11, 2025Jérémy H. Raymond, Zackie Aktary, Marie Pouteaux, Valérie Petit, Flavie Luciani, Maria Wehbe, Patrick Gizzi, Claire Bourban, Didier Decaudin, Fariba Nemati, et al.luc2 (from Photinus pyralis) was amplified by PCR from the pGL4.50[luc2/CMV/Hygro] vector (Promega, E1310) and flanking XhoI restriction sites were added. The digested PCR fragment was subcloned into the SalI

(2) Protein binding in an mRNA 5′-UTR sterically hinders translationRNAFebruary 1, 2025Simon Felder, Irma M. Nelson, Breanne M. Hatfield, Kevin M. WeeksSupplemental Material. For all dual-luciferase assays, the control plasmid was firefly pGL4.50 vector (E1310, Promega), and contained transfection carrier plasmid DNA (E4881, Promega). All plasmids were transformed

(3) Fast Ultrasound Scanning is a Rapid, Sensitive, Precise and Cost-Effective Method to Monitor Tumor Grafts in MiceJournal of Mammary Gland Biology and NeoplasiaDecember 1, 2024Sébastien Molière, Arthur Martinet, Amélie Jaulin, Massimo Lodi, Thien-Nga Chamaraux-Tran, Fabien Alpy, Guillaume Bierry, Catherine Tomasettomammalian expression, was amplified by PCR from the pGL4.50[luc2/CMV/Hygro] plasmid template (Cat.# E1310, Promega, Madison, WA). Flanking XhoI restriction sites were added for the subcloning into the SalI

(4) Establishment of Translational Luciferase-Based Cancer Models to Evaluate Antitumoral TherapiesInternational Journal of Molecular SciencesSeptember 27, 2024Martin R. Ramos-Gonzalez, Nagabhishek Sirpu Natesh, Satyanarayana Rachagani, James Amos-Landgraf, Haval Shirwan, Esma S. Yolcu, Jorge G. Gomez-Gutierrezper well in a 24-well plate, followed by transfection with the plasmid pGL4.50[luc2/CMV/Hygro] (Cat. E1310, Promega, Madison, WI, USA). Transfection was performed with jetPRIME transfection reagent (Polyplus-transfection,

(5) Orthotopic model for the analysis of melanoma circulating tumor cellsScientific ReportsApril 3, 2024Markéta Pícková, Zuzana Kahounová, Tomasz Radaszkiewicz, Jiřina Procházková, Radek Fedr, Michaela Nosková, Katarzyna Anna Radaszkiewicz, Petra Ovesná, Vítězslav Bryja, Karel Součekatmosphere and 95% relative humidity. The cells were transfected with the pGL4.50 (luc2/CMV/Hygro) Vector (E1310, Promega, Madison, WI) containing resistance to Hygromycin B to enable stable luciferase expression

(6) Transcriptomic analysis of the innate immune response to in vitro transfection of plasmid DNAMolecular Therapy - Nucleic AcidsMarch 14, 2023Eric Warga, Jared Anderson, Matthew Tucker, Emily Harris, Jacob Elmer35,051, respectively), while the luciferase expression plasmid pGL4.50 was purchased from Promega (cat# E1310). Cell lines Human PC-3 prostate cancer (Cat# CRL-1435), Jurkat T lymphoma (TIB-152), and HEK-293T

(7) Tumor Growth Suppression of Pancreatic Cancer Orthotopic Xenograft Model by CEA-Targeting CAR-T CellsCancers (Basel)January 18, 2023Osamu Sato, Takahiro Tsuchikawa, Takuma Kato, Yasunori Amaishi, Sachiko Okamoto, Junichi Mineno, Yuta Takeuchi, Katsunori Sasaki, Toru Nakamura, Kazufumi Umemoto, et al.hygromycin. The vector was prepared by introducing the GFP gene into the pGL4.50[luc2P/CMV/Hygro] vector (#E1310, Promega). 2.3. Analysis of CEA Expression on the Surface CEA expression on cell surfaces in all cell

(8) Inducible localized delivery of an anti-PD-1 scFv enhances anti-tumor activity of ROR1 CAR-T cells in TNBCBreast cancer research : BCRJune 3, 2022Harrasser M, Gohil SH, Lau H, Della Peruta M, Muczynski V, Patel D, Miranda E, Grigoriadis K, Grigoriadis A, Granger D, et al.(#17616), and firefly and Gaussia luciferase sequences were cloned from commercial vectors (Promega, #E1310, and ThermoScientific, #16146). Lentiviral production Functional lentiviral particles are generated

(9) Developmental maturation of the hematopoietic system controlled by a Lin28b-let-7-Cbx2 axisCell reportsApril 5, 2022Wang D, Tanaka-Yano M, Meader E, Kinney MA, Morris V, Lummertz da Rocha E, Liu N, Liu T, Zhu Q, Orkin SH, et al.paper,N/A ,, Recombinant DNA,, ,, pCW57.1,Gift from David Root,"Addgene plasmid # 41,393" pGL4.50,Promega,Cat#E1310 pNL3.1,Promega,Cat#N1031 pNL3.1-Erg,This paper,N/A pMSCV-neo let-7g,Kumar et al. (2007),Addgene plasmid

(10) Nonviral gene delivery to T cells with Lipofectamine LTXBiotechnology and BioengineeringApril 1, 2021Emily Harris, Devon Zimmerman, Eric Warga, Anil Bamezai, Jacob Elmervehicles in Jurkat cells used either the luciferase (luc2 gene)30 expression plasmid pGL4.50 (Promega, #E1310) or the EGFP expression plasmid pEF-GFP (Addgene, #11154). The pEF-Luc plasmid used in Figures 1 & 2

(11) Identification of Novel MeCP2 Cancer-Associated Target Genes and Post-Translational ModificationsFrontiers in OncologyDecember 10, 2020Isabel Castro-Piedras et al.generated using outward PCR method. Bioluminescent MDA-MB-468 Cells The pGL4.50[luc2/CMV/Hygro] plasmid (E1310) which encodes the luciferase reporter gene luc2 (Photinus pyralis) was purchased from Promega. MDA-MB-468

(12) Modeling Patient-Derived Glioblastoma with Cerebral OrganoidsCell reportsMarch 19, 2019Linkous A, Balamatsias D, Snuderl M, Edwards L, Miyaguchi K, Milner T, Reich B, Cohen-Gould L, Storaska A, Nakayama Y, et al.Blast (w510-5),"Campeau et al., 2009",Addgene Plasmid# 19069 pGL4.50[luc2/CMV/Hygro],Promega,Cat# E1310; N/A pLenti-C-tRFP,OriGene,Cat# PS100074 pLenti-C-TurboRFP-P2A-CLuc,Constructed in Lab,N/A Software

(13) Folate receptor 1 (FOLR1) targeted chimeric antigen receptor (CAR) T cells for the treatment of gastric cancerPloS oneJanuary 1, 2018Kim M, Pyo S, Kang CH, Lee CO, Lee HK, Choi SU, Park CH.human T cells were co-cultured with target cells (2×104) transfected with the luciferase gene (Cat. #E1310, Promega, USA) at indicated E/T ratios for 4 h. Luciferase activity was detected by the Bright-Glo Luciferase Assay System

(14) Identification of novel loci for the generation of reporter miceNucleic Acids ResearchApril 7, 2017Nicoletta Rizzi, Monica Rebecchi, Giovanna Levandis, Paolo Ciana, Adriana Maggicytomegalovirus immediate early promoter derived from pGL4.50 plasmid from Promega Corporation cod E1310) (Figure 1A, 1); however, based on our initial experience corroborated by data in the literature we

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