pGEM-7Zf(+) Vector from Promega

Product Specs
ItempGEM-7Zf(+) Vector
CompanyPromega
Price $390.00Supplier Page View Company Product Page
Catalog NumberP2251
HostBacteria
MCSApa I, Aat II, Sph I, Xba I, Xho I, EcoR I, Kpn I, Sma I, Csp45 I, Cla I, Hind III, BamH I, Sac I, BstX I and Nsi I
ResistanceAmpR
Origin of ReplicationpGEM®-3Zf(+)
Vector NamepGEM^®-7Zf(+)
Selectable MarkerBlue/White Screening
Quantity20microg
PromoterSP6 and T7 RNA polymerase promoters

Specifications/Features

Features:
• Blue/White Screening: Allows the easy identification of recombinant clones.
• Versatile: These vectors can be used for standard cloning, single-stranded DNA production and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
• Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

Vector Size:
2997 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Experimental horizontal transfer of phage-derived genes to Drosophila confers innate immunity to parasitoidsCurrent BiologyFebruary 3, 2025Rebecca L. Tarnopol, Josephine A. Tamsil, Gyöngyi Cinege, Ji Heon Ha, Kirsten I. Verster, Edit Ábrahám, Lilla B. Magyar, Bernard Y. Kim, Susan L. Bernstein, Zoltán Lipinszki, et al.pUAST::attB,97,RRID:DGRC_1419 Plasmid: pAG425-ccdB,101,Addgene #14153 Plasmid: pGEM-7zf(+),Promega,Catalog #P2251 Software and algorithms,Software and algorithms,Software and algorithms BLAST,75,https://blast.ncbi.nlm.nih.gov/Blast.cgi cd-hit

(2) LINE1-Mediated Reverse Transcription and Genomic Integration of SARS-CoV-2 mRNA Detected in Virus-Infected but Not in Viral mRNA-Transfected CellsVirusesFebruary 25, 2023Zhang L, Bisht P, Flamier A, Barrasa MI, Friesen M, Richards A, Hughes SH, Jaenisch R.(ATG) of the nucleocapsid ORF to the ending 25-nt poly-A was cloned into a pGEM-7Zf(+) vector (Promega; P2251) by XbaI (NEB; R0145L) and BamHI (NEB; R3136L) restriction cloning. This plasmid was linearized by BamHI

(3) OMP31 of Brucella melitensis 16M impairs the apoptosis of macrophages triggered by TNF-αExperimental and Therapeutic MedicineOctober 1, 2016Ke Zhang, Hui Wang, Fei Guo, Li Yuan, Wanjiang Zhang, Yuanzhi Wang, Chuangfu Chendigestion of the recombinants, the target fragments were introduced into a pGEM‑7zf (+) suicide vector (P2251; Promega Corp., Beijing, China). The sacB gene of Bacillus subtilis was amplified by PCR and inserted

(4) A complex containing LPP and α-actinin mediates TGFβ-induced migration and invasion of ErbB2-expressing breast cancer cellsJournal of cell scienceMay 1, 2013Ngan E, Northey JJ, Brown CM, Ursini-Siegel J, Siegel PM.#:MMM1013-98478393, Open Biosystems). Full length LPP was shuttled into SphI/XhoI sites of the pGEM7zf vector (cat #:P2251, Promega) as a SalI/SphI fragment. The vector was then enzymatically digested with PshAI/ApaI and the

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pGEM-7Zf(+) Vector

pGEM-7Zf(+) Vector from Promega

Description

Specifications/Features

Promega

Citations (4)

Reviews

pGEM-7Zf(+) Vector from Promega

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