pSV-beta-Galactosidase Control Vector from Promega

Product Specs
ItempSV-beta-Galactosidase Control Vector
CompanyPromega
Price Supplier Page View Company Product Page
Catalog NumberE1081
HostMammalian
Vector NamepSV-beta-Galactosidase Control Vector
Quantity20microg
PromoterSV40 early promoter and enhancer
Reporterß-Galactosidase
Molecular Weight~59kDa (CDK); ~60kDa (p35)

Specifications/Features

Vector Size:
6,820 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) CI-994 is a dual modulator of class I HDACs and Wnt/β-catenin signaling for the treatment of Alzheimer's diseaseAlzheimer's Research & TherapyFebruary 7, 2026Wenyan Lu, Keiji Kawatani, Yingxue Ren, Toshihiko Nambara, Lin Jia, Suren Jeevaratnam, Eunmi Lee, Paula Rodriguez Martinez, Taha Izhar, Ni Wang, et al.described previously [26]. A β-galactosidase-expressing vector was purchased from Promega (cat. no. E1081). The luciferase assay system (cat. no. E1500) and β-galactosidase enzyme assay system (cat. no. E2000)

(2) Circular RNA circAtxn10 regulates skeletal muscle cell differentiation by targeting miR-143-3p and Chrna1The Korean Journal of Physiology & PharmacologySeptember 1, 2025Nakwon Choe, Anna Jeong, Hosouk Joung, Dongtak Jeong, Young-Kook Kim, Hyun Kook, Duk-Hwa KwonLuciferase Assay System (E1500, Promega) following the manufacturer's protocols. The pSV-β-Galactosidase Control Vector (E1081, Promega) was also co-transfected to normalize the luciferase signal with β-Galactosidase Enzyme Assay

(3) Identification of New Key Players for Ferrous Iron Export in the Asymmetric Inner Gate of Human Ferroportin 1FASEB JJuly 31, 2025Marlène Le Tertre, Ahmad Elbahnsi, Cécile Ged, Kevin Uguen, Isabelle Gourlaouen, Claude Férec, Chandran Ka, Gérald Le Gac, Isabelle CallebautpcDNA3.1_FPN‐V5 construct was co‐delivered with the pSV‐β‐Galactosidase control vector (Promega Cat# E1081). After being washed, the cells were harvested with trypsin, mixed with liquid scintillation fluid (Ultima

(4) Species-selective activation of a β-galactosidase fluorogenic probeSci RepJuly 2, 2025Kaja Jaskot, Angelika Mielcarek, Łukasz Popenda, Ahmet Kertmen, Bartosz F. Grześkowiak, Jan Barciszewski, Patrick M. Perriguefigures were generated using IDEAS software41. DNA transfection pSV-β-Galactosidase Control vector (E1081) containing the lacZ gene was obtained from Promega (Madison, WI, USA). HEK293T cells were seeded into

(5) The extracellular matrix protein type I collagen and fibronectin are regulated by β-arrestin-1/endothelin axis in human ovarian fibroblastsJ Exp Clin Cancer ResFebruary 21, 2025Ilenia Masi, Flavia Ottavi, Valentina Caprara, Danila Del Rio, Martina Kunkl, Francesca Spadaro, Valerio Licursi, Loretta Tuosto, Anna Bagnato, Laura Rosano'µg COL1A1-luc vector or empty control vectors (Promega) with 250 ng pSV-β-galactosidase vector (Cat# E1081 Promega). Cells in different experimental conditions were plated in a 6-well plate until they reached

(6) The O-glycosyltransferase C1GALT1 promotes EWSR1::FLI1 expression and is a therapeutic target for Ewing sarcomaNat CommunFebruary 2, 2025Shahid Banday, Alok K. Mishra, Romana Rashid, Tianyi Ye, Amjad Ali, Junhui Li, Jason T. Yustein, Michelle A. Kelliher, Lihua Julie Zhu, Sara K. Deibler, et al.and PCR amplification of the SV40 polyA terminator from plasmid pSV-β-Galactosidase (Promega, Cat# E1081); the PCR products were denatured and annealed, PCR amplified, digested and ligated downstream of tdTomato-2A

(7) Disruption of cellular plasticity by repeat RNAs in human pancreatic cancerCellDecember 12, 2024Eunae You, Patrick Danaher, Chenyue Lu, Siyu Sun, Luli Zou, Ildiko E. Phillips, Alexandra S. Rojas, Natalie I. Ho, Yuhui Song, Michael J. Raabe, et al.purchased from Horizon Discovery (Dharmacon). pSV-beta-galactosidase control vector (Promega, cat# E1081) and Human V5-IRF3-pcDNA3 (Addgene, cat# 32713) were transfected in CAF-1 using Lipofectamine™ 2000 Transfection Reagent

(8) β-Galactosidase- and Photo-Activatable Fluorescent Probes for Protein Labeling and Super-Resolution STED Microscopy in Living CellsMoleculesJuly 30, 2024Taukeer A. Khan, Stefan Stoldt, Mariano L. Bossi, Vladimir N. Belov, Stefan W. Hellcommercially available plasmid pSV-β-galactosidase control vector (Promega, Fitchburg, WI, USA, Cat.Nr. E1081) was used for transfection. Here, the expression of the lacZ gene, which encodes the β-Gal, is driven

(9) Semaphorin Receptors Antagonize Wnt Signaling Through Beta-Catenin DegradationbioRxiv : the preprint server for biologyMay 29, 2024Hoard TM, Liu K, Cadigan KM, Giger RJ, Allen BL.luciferase reporter constructs and β-galactosidase transfection control (pSV-β-galactosidase; Promega, E1081). Transfections were performed using Lipofectamine 2000 (Invitrogen, 11668) and Opti-MEM reduced serum

(10) Phloroglucinol Enhances Anagen Signaling and Alleviates H2O2-Induced Oxidative Stress in Human Dermal Papilla CellsJournal of Microbiology and BiotechnologyApril 28, 2024Seokmuk Park, Ye Jin Lim, Hee Su Kim, Hee-Jae Shin, Ji-Seon Kim, Jae Nam Lee, Jae Ho Lee, Seunghee Baeplates and incubated at 37°C for 24 h. The cells were transfected with pSV-β-galactosidase plasmid (#E1081; Promega, USA) and TCF/LEF response element-driven luciferase reporter plasmid (#12456; Addgene, USA)

(11) Depletion of JunB increases adipocyte thermogenic capacity and ameliorates diet-induced insulin resistanceNature MetabolismJanuary 8, 2024Xing Zhang, Xiaofeng Ding, Chunqing Wang, Que Le, Dandan Wu, Anying Song, Guixiang Huang, Liping Luo, Yan Luo, Xin Yang, et al.with constructs that encode PGC-1α, JunB, luciferase reporter and pSV-β-galactosidase control vector (E1081, Promega) with Lipofectamine 3000 Transfection Reagent (L3000–008, Thermo Fisher). After 48 h, cells

(12) In vitro hair growth-promoting effects of araliadiol via the p38/PPAR-γ signaling pathway in human hair follicle stem cells and dermal papilla cellsFrontiers in pharmacologyJanuary 1, 2024Park S, Park HW, Seo DB, Yoo DS, Bae S.conducted using previously described methods (Park et al., 2024). The pSV-β-galactosidase plasmid (#E1081) was sourced from Promega, whereas the Gli response element-driven luciferase reporter plasmid (#113712),

(13) A novel splice variant of the human MSTN gene encodes a myostatin-specific myostatin inhibitorJournal of cachexia, sarcopenia and muscleOctober 1, 2023Maeta K, Farea M, Nishio H, Matsuo M.before transfection with the SBE4‐Luc luciferase reporter gene and pSV‐β‐galactosidase control vector (#E1081, Promega, Madison, WI, USA) and either the empty vector (pcDNA3.1(+)) or pcDNA3.1(+) harbouring pro‐myostatin

(14) Remodeling of the tumor microenvironment through PAK4 inhibition sensitizes tumors to immune checkpoint blockadeCancer Res CommunOctober 1, 2022Abril-Rodríguez G, Torrejon DY, Karin D, Campbell KM, Medina E, Saco JD, Galvez M, Champhekar AS, Perez-Garcilazo I, Baselga-Carretero I, et al.activity assay were performed as described previously (13) using pSV-β-galactosidase control vector (PR-E1081, Promega), pTopflash (Addgene, catalog no. 12456) and mouse recombinant Wnt-3a (R&D Systems). For luciferase

(15) Plexins promote Hedgehog signaling through their cytoplasmic GAP activityeLifeSeptember 28, 2022Pinskey JM, Hoard TM, Zhao XF, Franks NE, Frank ZC, McMellen AN, Giger RJ, Allen BL.luciferase reporter construct and beta-galactosidase transfection control (pSV-β-galactosidase; Promega, E1081). Transfections were performed using Lipofectamine 2000 (Invitrogen, 11668) and Opti-MEM reduced serum

(16) Mutated KLF4(K409Q) in meningioma binds STRs and activates FGF3 gene expressioniScienceAugust 19, 2022Alla V. Tsytsykova, Graham Wiley, Chuang Li, Richard C. Pelikan, Lori Garman, Francis A. Acquah, Blaine H.M. Mooers, Erdyni N. Tsitsikov, Ian F. DunnSigma-Aldrich). Transfection efficiencies were normalized on co-transfected pSV-β-Galactosidase Control Vector (E1081, Promega). β-Gal levels were measured using β-Gal Reporter Gene Assay (11758241001, Sigma-Aldrich).

(17) Organoid-derived intestinal epithelial cells are a suitable model for preclinical toxicology and pharmacokinetic studiesiScienceJuly 15, 2022Yu Takahashi, Makoto Noguchi, Yu Inoue, Shintaro Sato, Makoto Shimizu, Hirotatsu Kojima, Takayoshi Okabe, Hiroshi Kiyono, Yoshio Yamauchi, Ryuichiro SatoDNA ,, p3xFLAG-CMV-10,Sigma-Aldrich,E7658 p3xFLAG-CMV-10-hSGLT1,This paper,N/A pNL1.2,Promega,N1011 pNL1.2-hCYP3A4,This paper,N/A pSV40-β-gal,Promega,E1081 pCAG-HIVgp,RIKEN BioResource Research Center,N/A pCMV-VSV-G-RSV-Rev,RIKEN BioResource Research Center,N/A CS-UbC-hSox2-IRES2-Venus,RIKEN

(18) An Antisense Oligonucleotide against a Splicing Enhancer Sequence within Exon 1 of the MSTN Gene Inhibits Pre-mRNA Maturation to Act as a Novel Myostatin InhibitorInternational journal of molecular sciencesApril 30, 2022Maeta K, Farea M, Nishio H, Matsuo M.before transfection with the SBE4-Luc luciferase reporter gene, a pSV-β-galactosidase control vector (#E1081, Promega, Madison, WI, USA), and either ASO or water. The cells were washed with phosphate-buffered

(19) Dihydroartemisinin attenuates osteoclast formation and bone resorption via inhibiting the NF‑κB, MAPK and NFATc1 signaling pathways and alleviates osteoarthritisInternational journal of molecular medicineJanuary 1, 2022Ding D, Yan J, Feng G, Zhou Y, Ma L, Jin Q.51985091; Thermo Fisher Scientific, Inc.). The pSV-β-galactosidase control vector (0.5 µg, cat. no. E1081; Promega Corporation) was co-transfected, according to the manufacturer's instructions, for normalization.

(20) miR-221 confers lapatinib resistance by negatively regulating p27(kip1) in HER2-positive breast cancerCancer scienceOctober 1, 2021Huynh TK, Huang CH, Chen JY, Yao JH, Yang YS, Wei YL, Chen HF, Chen CH, Tu CY, Hsu YM, et al.the KpnI/SacI sites. pGL4.22‐p27 3′UTR was co‐transfected with pSV‐β‐galactosidase control vector (E1081, Promega Corporation) into the indicated cell lines using the TransIT‐X2 transfection reagent (MIR6000,

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