pSP72 Vector from Promega

Product Specs
ItempSP72 Vector
CompanyPromega
Price $65.00Supplier Page View Company Product Page
Catalog NumberP2191
HostBacteria
PromoterSP6 and T7 RNA polymerase promoters
Vector NamepSP72
Quantity20microg
MCSXho I, Pvu II, Hind III, Sph I, Pst I, Sal I, Acc I, Xba I, BamH I, Sma I, Kpn I, Sac I, EcoR I, Cla I, EcoR V and Bgl II
ResistanceAmpR

Specifications/Features

Vector Size:
2462 bp

Features:
• Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
• Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Protocol for assessing mitochondrial cholesterol transport and protein molten globule state in steroidogenic and nonsteroidogenic systemsSTAR ProtocolsSeptember 1, 2025Himangshu S. Bose, Mahuya Bose, Randy M. WhittalBluescript-KS,Creative Biogene,VET1448 (Originally obtained from Stratagene) Plasmid pSP72,Promega,Cat#P2191 ,, Biological samples,Biological samples,Biological samples ,, "University of Florida, Veterinary

(2) Evaluation of Immunogenicity of an Orf Virus Vector-Based Vaccine Delivery Platform in SheepVaccines (Basel)June 11, 2025Sean R. Wattegedera, Jackie Thomson, Lesley Coulter, Ann Wood, Rebecca K. McLean, Holly Hill, Cameron Cunnea, Karen Snedden, Ann Percival, Javier Palarea-Albaladejo, et al.117 and complete genes 118 and 119) into commercial cloning vector pSP72 (Promega, Southampton, UK, #P2191) by PCR, targeting appropriate restriction enzyme sites and using the cloning protocols outlined in

(3) Mechanisms underlying Myosin 10′s contribution to the maintenance of mitotic spindle bipolarityMolecular Biology of the CellFebruary 1, 2024Yang-In Yim, Antonio Pedrosa, Xufeng Wu, Krishna Chinthalapudi, Richard E. Cheney, John A. Hammerand an XhoI site. For cloning convenience, ∼20 bp taken from the backbone of plasmid pSP72 (Promega, P2191) was added onto the outside end of both 5′ and 3′HDRs. Below is the complete gBlock sequence (the guide

(4) Myosin 10 uses its MyTH4 and FERM domains differentially to support two aspects of spindle pole biology required for mitotic spindle bipolaritybioRxivJune 20, 2023Yang-In Yim, Antonio Pedrosa, Xufeng Wu, Krishna Chinthalapudi, Richard E. Cheney, John A. Hammerand an XhoI site. For cloning convenience, ~20 bp taken from the backbone of plasmid pSP72 (Promega, P2191) was added onto the outside end of both 5’ and 3’HDRs. Below is the complete gBlock sequence (the guide

(5) Enhancement of Vivid-based photo-activatable Gal4 transcription factor in mammalian cellsCell Structure and FunctionFebruary 8, 2023Shinji C. Nagasaki, Tomonori D. Fukuda, Mayumi Yamada, Yusuke III Suzuki, Ryo Kakutani, Adam T. Guy, Itaru ImayoshiUAS-Ub-Luc2-Hes1 3' UTR sequence was amplified by PCR method and inserted into pSP72 vector (Promega, P2191). To construct the 5x UAS-Achilles-NLS-PEST-Hes1 3' UTR plasmid, PB-TRE-dCas9-VPR (Addgene plasmid #63800)

(6) Generation of a Novel Oncolytic Vaccinia Virus Using the IHD-W StrainHuman gene therapyMay 1, 2021Shin J, Hong SO, Kim M, Lee H, Choi H, Kim J, Hong J, Kang H, Lee E, Lee S, et al.IHD-W vaccinia virus genome by homologous recombination were constructed based on the pSP72 vector (P2191; Promega, WI). Approximately 1 kb of both flanking regions of the target genes bearing reporter gene(s)

(7) Mesenchymal stem cells injected into carotid artery to target focal brain injury home to perivascular spaceTheranosticsMay 17, 2020Anna Andrzejewska, Sylwia Dabrowska, Blazej Nowak, Piotr Walczak, Barbara Lukomska, Miroslaw Janowskias we previously described 12. Briefly, the cDNA for ITGA4 gene was inserted into the pSP72 vector (P2191-Promega) and served as a template for in vitro production of mRNA capped with an anti-reverse-cap-analogue

(8) Translation, but not transfection limits clinically relevant, exogenous mRNA based induction of alpha-4 integrin expression on human mesenchymal stem cellsScientific ReportsDecember 1, 2017Adam Nowakowski, Anna Andrzejewska, Johannes Boltze, Franziska Nitzsche, Li-li Cui, Jukka Jolkkonen, Piotr Walczak, Barbara Lukomska, Miroslaw Janowskicommercially designed vector for mRNA synthesis in vitro (2.4 kbp pSP72 plasmid) was obtained from Promega (P2191, Promega). pITGA4 and pITGA4(IRES)eGFP plasmids were produced in our lab (The Johns Hopkins University

(9) Repair of Chromosomal RAG-Mediated DNA Breaks by Mutant RAG Proteins Lacking Phosphatidylinositol 3-Like Kinase Consensus Phosphorylation SitesThe Journal of ImmunologyAugust 15, 2011Eric J. Gapud, Baeck-Seung Lee, Grace K. Mahowald, Craig H. Bassing, Barry P. Sleckmanproteins N-terminal FLAG-tagged versions of RAG1 and RAG2 were generated in the pSP72 shuttle vector (Promega, P2191). To this end we first generated a version of pSP72 (pSP72-FLAG) that contained a linker composed of

(10) JUN-CCAAT/Enhancer-Binding Protein Complexes Inhibit Surfactant-Associated Protein B Promoter ActivityAmerican Journal of Respiratory Cell and Molecular BiologyAugust 1, 2011Kiflai Bein, Hayley Leight, George D. Leikauf12550 and 12557, respectively; Addgene, Cambridge, MA) and negative control pSP72 (catalogue number P2191; Promega) were linearized to produce in vitro translated proteins (catalogue number L4610; Promega).

(11) Regulation of human bone marrow stromal cell proliferation and differentiation capacity by glucocorticoid receptor and AP-1 crosstalkJournal of Bone and Mineral ResearchOctober 1, 2010Iván Cárcamo-Orive, Ainhoa Gaztelumendi, Jesús Delgado, Naiara Tejados, Akaitz Dorronsoro, Jon Fernández-Rueda, Daniel J Pennington, César Triguerosc-Jun sequence NM_000228. c-Jun cDNA was excised and cloned into the pSP72 vector (Promega Plasmid P2191, http://www.promega.com) using EcoRI and BamH1. EcoRV and PvuII were used to get c-Jun cDNA from pSP72,

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pSP72 Vector

Description

Specifications/Features

Promega

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Reviews

pSP72 Vector from Promega

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