pGEM-3Z Vector from Promega

Product Specs
ItempGEM-3Z Vector
CompanyPromega
Price $66.00Supplier Page View Company Product Page
Catalog NumberP2151
HostBacteria
Origin of ReplicationpUC18
Quantity20microg
PromoterSP6 and T7 promoters
ResistanceAmpR
MCSMultiple cloning site provides a selection of restriction sites for cloning.

EcoR I, Sac I, Kpn I, Ava I, Xma I, Sma I, BamH I, Xba I, Sal I, Acc I, Hinc II, Pst I, Sph I, Hind III
Selectable MarkerBlue/white screening for recombinants
Vector NamepGEM^®-3Z

Specifications/Features

Vector Size:
2743 bp

Features:
• Blue/White Screening: Allows the easy identification of recombinant clones.
• Versatile: This vector can be used for standard cloning and in vitro transcription from SP6 and T7 RNA polymerase promoters flanking the multiple cloning region.
• Convenient: Multiple cloning site provides a selection of restriction sites for cloning.

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Disruption of P. falciparum amino acid transporter elevates intracellular proline and induces resistance to Prolyl-tRNA synthetase inhibitorsCell Chemical BiologyOctober 16, 2025Selina Bopp, Lọla Fagbami, Amy Deik, Claudia Taccheri, Akansha Pant, Madeline Luth, Daisy Chen, Mark A. Tye, Imran Ullah, Johannes Kreuzer, et al.Technologies,N/A Recombinant DNA,Recombinant DNA,Recombinant DNA pL-6_eGFP,Ghorbal et al.39,N/A pGEM-3Z,Promega,Cat# P2151 pGEMhdfr+,This paper,N/A pDC2-cam-Cas9-U6-hDHFR,Ng et al.40,N/A Software and algorithms,Software

(2) Stem loop binding protein promotes SARS-CoV-2 replication via -1 programmed ribosomal frameshiftingSignal Transduct Target TherJune 13, 2025Tanxiu Chen, Ruimin Zhu, Tingfu Du, Hao Yang, Xintian Zhang, Zhixing Wang, Yong Zhang, Wenqi Quan, Bin Yin, Yunpeng Liu, et al.Then, identical quantities of the samples were incubated with BeyoMag™ Streptavidin Magnetic Beads (P2151, Beyotime) for 6 h at 4 °C. The enrichment of proteins bound by probes was examined. The sequence of

(3) Upregulation of protein O-GlcNAcylation levels promotes zebrafish fin regenerationMolecular & Cellular ProteomicsMarch 4, 2025Liyuan Jia, Hanxue Zheng, Juantao Feng, Yi Ding, Xiaotian Sun, Yuan Yu, Xue Hao, Junxiang Wang, Xinyu Zhang, Yuanfeng Tian, et al.overnight at 37 °C by trypsin (Promega; Cat# VA9000) at 1:100 (w/w). Using a magnetic frame (Beyotime, Cat# P2151), peptides were isolated and collected by C18 column (Waters Corp, Cat# WAT036905) (22, 23) (Supplemental

(4) Targeting SNORA38B attenuates tumorigenesis and sensitizes immune checkpoint blockade in non-small cell lung cancer by remodeling the tumor microenvironment via regulation of GAB2/AKT/mTOR signaling pathwayJournal for immunotherapy of cancerMay 1, 2022Zhuo Y, Li S, Hu W, Zhang Y, Shi Y, Zhang F, Zhang J, Wang J, Liao M, Chen J, et al.endonucleases EcoRI (R6011, Promega) and SalI (R6051, Promega) were used to linearized the pGEM-3Z vector (P2151, Promega), then pGEM-3Z-SNORA38B plasmid was constructed. Biotinylated sense and anti-sense SNORA38B

(5) Structural basis of TRPC4 regulation by calmodulin and pharmacological agentseLifeNovember 25, 2020Deivanayagabarathy Vinayagam et al.section for details),"Vinayagam et al., 2018 PMID:29717981",, Recombinant DNA reagent,pGEMHE 22,Promega,P2151,pGEMHE 22 is a derivative of pGEM3z "Chemical compound, drug",(-)-Englerin A,Carl Roth,Cat.No.6492.1, "Software,

(6) FOXC1-mediated LINC00301 facilitates tumor progression and triggers an immune-suppressing microenvironment in non-small cell lung cancer by regulating the HIF1α pathwayGenome medicineSeptember 2, 2020Sun CC, Zhu W, Li SJ, Hu W, Zhang J, Zhuo Y, Zhang H, Wang J, Zhang Y, Huang SX, et al.exposed to qPCR analysis. RNA pull-down assays LINC00301 was constructed into the pGEM-3Z vector (P2151, Promega) using KpnI (R3142, New England Biolabs) and NheI (R3131, New England Biolabs) restriction

(7) Expression of human nPTB is limited by extreme suboptimal codon contentPloS oneMarch 12, 2008Robinson F, Jackson RJ, Smith CW.nucleotides of the mammalian β-globin 5′ UTR into the Eco RI/Kpn I sites of pGEM3Z (Promega Cat. no. P2151), providing an adequate distance between the transcription and translation start sites to allow efficient

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pGEM-3Z Vector

pGEM-3Z Vector from Promega

Description

Specifications/Features

Promega

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Reviews

pGEM-3Z Vector from Promega

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