pRL-TK Vector from Promega

Product Specs
ItempRL-TK Vector
CompanyPromega
Price $0.00Supplier Page View Company Product Page
Catalog NumberE2241
HostMammalian
PromoterHSV TK promoter
Vector NamepRL-TK
Quantity20microg
ReporterRenilla luciferase
ResistanceAmpR
Molecular Weight~73kDa
Selectable MarkerAmpicillin

Specifications/Features

Vector Size:
4045 bp

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Promega

2800 Woods Hollow Rd

Madison, WI 53711

United States

Phone: Tel: +1 (608) 274-4330
Fax: +1 (608) 277-2516

Email: custserv@promega.com
techserv@promega.com

Company Profile

Website: http://www.promega.com

(1) Loss of SETD2-mediated H3K36me3 Drives CD8+ T Cell Exhaustion in Lung Cancer through CXCL-dependent PMN-MDSC Recruitment and Arginine DeprivationResearch (Wash D C)August 5, 2026Xuyu Gu, Junjie Zhu, Tao Ge, Keyi Chen, Yang Yang, Kaiqi Jin, Xinnan Xuconfluence). Cells were cotransfected with 1 μg of promoter–reporter plasmid and 50 ng of pRL-TK (#E2241, Promega) as internal control. Medium was replaced 6 h later, followed by another 24 h. Cells were lysed

(2) Loss of NCCRP1 overcomes immune evasion in lung adenocarcinomaJ Immunother CancerAugust 5, 2026Lu Liu, Shihao Qi, Liye Shao, Junyang Cai, Qingqing Zeng, Xu Jiang, Fang Ma, Weiwei Lin(China). SgCtrl or sgNccrp1 LLC cells were co-transfected with each reporter plasmid and the pRL-TK (E2241, Promega) plasmid using the liposomal transfection reagent GTR. After 48 hours of transfection, firefly

(3) Transcription Factor MAX Regulates Liver Cancer Cell Growth, Migration, Invasion, and Epithelial–Mesenchymal Transition by Promoting SF3A3 ExpressionWorld Journal of OncologyAugust 1, 2026Gui Zhou, Mei Yu Dai, Xiang Chen, Li Ming Liu, Fa Quan Linhairpin RNA (shRNA) targeting MAX (100 ng), and the Renilla luciferase control vector pRL-TK (10 ng, Cat# E2241, Promega) using Lipofectamine 3000 (Thermo Fisher). After 48 h, the cells were lysed and luciferase

(4) TBX20 promotes doxorubicin resistance in breast cancer cells through enhanced ABCC1 expression and inhibition of mitophagyPLoS OneJuly 17, 2026Shuai Nie, Dejian Liu, Jun Hu, Yu Gan, Liangliang Mireagent (Invitrogen, L3000015) at a 1:3 plasmid-to-reagent ratio. A control plasmid, pRL-TK (Promega, E2241), expressing Renilla luciferase, was co-transfected at 20 ng per well to normalize for transduction

(5) The STAT3-CCND2 Axis Drives a Proliferative Metaplastic Precursor Population in Gastric Intestinal MetaplasiaJ Cell Mol MedJuly 15, 2026Fazhan Li, Huijuan Wen, Feifei Ren, Pengyuan Zheng, Simeng LiuTransfection efficiency was monitored by co‐transfection of a Renilla luciferase control plasmid (pRL‐TK, Promega, USA, E2241) [41]. Luciferase activities were quantified 48 h post‐transfection using a dual‐luciferase assay system

(6) A membrane homeostatic response to lipid overload coordinates fatty acid metabolismbioRxivJuly 6, 2026Xiaojun Xiang, Yohannes A. Ambaw, Ozlem Tok, Sheng Hui, Wei-chun Tang, Arda Mizrak, Qingyue Zhang, Luke Cohen-Abeles, Robert V. Farese, Tobias C. WaltherPPARG, OHu24140D). pGL4.29[luc2P/CRE/Hygro] and pRL-TK vectors were purchased from Promega E8471 and E2241). The luciferase reporter containing the PPRE from the human CPT1A promoter (PPRE in hCPT1A) was generated

(7) IGFL2 suppresses gastric cancer cell sensitivity to docetaxel by activating the Toll-like receptor signaling pathwayiScienceJune 19, 2026Wei Huang, Zhirui Tao, Xiaolin Xu, Huangming Cao, Li Cheng, Jin LiDNA ,, pGL3-TLR4 promoter reporter plasmid,GeneChem,N/A pRL-TK Renilla luciferase plasmid,Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism 9,GraphPad

(8) Microsomal triglyceride transfer protein restricts steroid production in Leydig cells by regulating SREBP2iScienceJune 19, 2026Atrayee Chattopadhyay, Munichandra Babu Babu Tirumalasetty, Thomas Palaia, Bhargavi Gangula, Rachel Ruoff, Qing Robert Miao, M. Mahmood HussainS1 ,, Recombinant DNA,Recombinant DNA,Recombinant DNA ,, pSynSRE-T-Luc,Addgene,Cat #60444 pRL-TK,Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, GraphPad Prism,GraphPad

(9) Gemcitabine activates the Hippo signaling pathway and suppresses tumor growth by stabilizing large tumor suppressor kinase 2 through the hypoxia-inducible factor 1-alpha/ubiquitin protein ligase E3 component N-recognin 5 axisJ Cell Commun SignalJune 17, 2026Jin Zhang, Lixia Xu, Kai Guo, Weijia Dong, Xin Dai, Donghua Guco‐transfected with 400 ng of reporter plasmid, 50 ng of the Renilla luciferase internal control plasmid pRL‐TK (E2241, Promega, USA), and 400 ng of oe‐HIF1A or an equivalent amount of empty vector. After 24 h, cells were

(10) PRDM1 Drives a TIM3+ Macrophage Immunosuppressive Niche via LGALS9 Signaling in Prostate Cancer ProgressionOncology ResearchJune 16, 2026Yangyang Zhang, Ilyar Mamtili, Yonghao Chen, Guangjie Ji, Chaozhao LiangRenilla luciferase internal control plasmid used for normalization was the pRL-TK Vector (Promega, Cat# E2241, Madison, WI, USA). For each well of a 24-well plate, cells were co-transfected with 400 ng of HAVCR2

(11) CL-316243 facilitates stable atherosclerotic plaque phenotypes in association with suppression of perivascular adipose tissue ferroptosis via upregulating C/EBPβRedox BiolJune 13, 2026Yuanqing Jiang, Yi Li, Kefan Ma, Suxiang Guo, Nachuan Liao, Jiayi Zhou, Junbo Chen, Ruizhe Ren, Yaohui Kou, Jinying Li, et al.1 μg of GPX4 plasmid (WT or Mutant 1-5), 1 μg of empty vector or C/EBPβ and 0.2 μg pRL-TK (Promega, E2241). Cells were cultured for 48 h and detected using the Dual Luciferase Reporter Gene Assay Kit (YEASEN,

(12) UCHL3 Regulates Subgenomic Flaviviral RNA Condensates to Promote Virus PropagationAdvanced ScienceJune 3, 2026Oscar Trejo‐Cerro, Anna Beekmayer‐Dhillon, Qi Wen Teo, Lewis Siu, Ming Yuan Li, Kate J. Heesom, Adán Pinto‐Fernández, Sumana Sanyalco‐transfected with the pISRE‐Luc reporter plasmid and the Renilla luciferase control plasmid pRL‐TK (Promega, E2241; 25 ng/well) as an internal normalization control, together with either the ZIKV sfRNA‐BoxB construct

(13) CEBPG‐Mediated Palmitic Acid Adaptation of Cancer‐Associated Fibroblasts Drives Metastasis of Oral Squamous Cell CarcinomaAdvanced ScienceJune 2, 2026Yiling Duan, Yitong Li, Yufei Wu, Xiao Yang, Rui Li, Hui Zhao, Zhengjun Shangstandard PCR‐cloning. 293T cells were co‐transfected with the luciferase construct and pRL‐TK (Promega, E2241) under conditions with or without transfection of pcDNA3.1‐CEBPG. Detection was performed using the

(14) Hesperetin-Black Phosphorus Nanosheets Targeting TAMs Induce Ferroptosis and Reverse Protumoral Polarization to Remodel the Immune MicroenvironmentMaterials Today BioMay 29, 2026Liang Feng, Huan Wang, Zuodi Liang, Ying Tian, Yang Guo, Zhuo Liwere co-transfected with these constructs (1 μg) and the pRL-TK Renilla reference plasmid (0.1 μg, E2241, Promega) employing Lipofectamine 3000 (Thermo Fisher Scientific, USA). After a 48-h incubation, cell

(15) Equine infectious anemia virus blocks interferon responses through Rev-mediated activation of the stress granule-PKR-eIF2α pathwayPLoS PathogMay 21, 2026Huiling Ren, Kewei Chen, Bingqian Zhou, Weiguo Zhang, Xue-Feng Wang, Xiaojun Wang(Sigma-Aldrich, E7658). The pEGFP-N1 vector (HG-VYC0086) was purchased from Clontech. The pRL-TK vector (E2241) was purchased from Promega. The mutants of Rev and G3BP1 were generated using PCR-based site-directed mutagenesis

(16) CDK4 Mediates Cisplatin Resistance in Renal Cell Carcinoma (RCC) Cells by Regulating the ASH1L-CTR1 AxisOncology ResearchMay 21, 2026Wenjian Zeng, Xianglong Li, Hao Cai, Qingyu Zhou, Shuangshuang Sun, Pingping Li, Sunlong Li, Zhi Chenof the internal reference vector pRL-TK (containing the Renilla luciferase gene, Promega, Cat. No.: E2241, Madison, USA) to correct transfection efficiency, 2 μL of Lipofectamine 3000 transfection reagent (Thermo

(17) APOE+ macrophages induce tumor cell metastatic characteristics via TNFSF12/TNFRSF12A signaling, correlating with poor patient prognosisCancer Cell InternationalMay 20, 2026Chuanjun Song, Hongyu Sun, Wenyuan Gan, Quanzhong Liu, Lingxiang Wu, Mengyan Zhu, Lingjun Zhucorresponding control vector, and the pRL-TK Renilla luciferase control reporter vector (Promega, Catalog #: E2241) as an internal control. Luciferase activity was measured 24–48 h after transfection using the Dual-Luciferase Reporter Assay System

(18) Linalool targets NR3C2 to inhibit NF-κB-mediated gastric cancer progressionCell DivisionMay 18, 2026Ying Hu, Shunfeng Chen, Yu Wang, Ling Liu, Chaoyi Zhang, Hong Chen, Yuanyuan Duanmu, Zhaotian Su, Jun Maassay, the NFKBIZ promoter reporter plasmid and the pRL Renilla Luciferase Control Reporter Vector (E2241, Promega) were co-transfected into each well. For the assessment of NF-κB transcriptional activity,

(19) Nuclear factor IX promotes endometriosis progression through transcriptional activation of tetraspanin-2J Mol Med (Berl)May 16, 2026Yuqi Liu, Qing Xue, Jingwen Zhu, Cheng Zeng, Xin Li, Jing Shang, Jie Li, Peili Wuluciferase reporter construct, along with 90 ng of Renilla luciferase reporter plasmid pRL-TK (Promega, E2241) as an internal control. Cells were gathered 48 h post transfection, and the luciferase assay system

(20) Prenatal bisphenol A exposure reprograms SF1-lactylation pathways to promote endometriosis susceptibilityiScienceMay 15, 2026Xiaohan Liu, Yanxia Fu, Liuxuan Huang, Donghan Li, Paul Yao, Liqin Zengluciferase reporter vector,Promega,Cat# E1751 Renilla luciferase control vector (pRL-TK),Promega,Cat# E2241 ,, Software and algorithms,Software and algorithms,Software and algorithms ,, ImageJ software,National

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pRL-TK Vector

pRL-TK Vector from Promega

Description

Specifications/Features

Promega

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Reviews

pRL-TK Vector from Promega

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