Daniel Hamilton

Graduate Student
UC San Diego NanoEngineering Department

Articles by Daniel Hamilton

  • Equipment User Review

    Good 96-Well Microplate

    July 15, 2022
    I used these microplates several times with different cell lines. I have never had any problem or contamination. I really ... read more
  • Equipment User Review

    Great 5 mL Pipettes

    July 15, 2022
    I always used these pipettes for my cell culture experiments. I have never had any problem or contamination. I really appreciate ... read more
  • Equipment User Review

    High Quality 12-Well Plate

    July 15, 2022
    I used these plates for several experiments. I appreciate the quality of the material and I have never had any contamination. ... read more
  • Equipment User Review

    High Quality Cell Culture Flasks

    July 15, 2022
    I used these cell culture flask with many different cell lines. I have never had any problems. I usually seed my cells with 12ml ... read more
  • Antibody User Review

    Great EGF Receptor Antibody for Western Blot

    July 12, 2022
    I used A431 as cell type. The cells were seeded with starvation medium and treated for 24 hours. I used rabbit GE as secondary ... read more
  • Antibody User Review

    Great Results With Western Blot GFP

    July 12, 2022
    This antibody has been working well for western blots, I have never had a problem with it. I use a 1:1000 dilution in BSA. Cell ... read more
  • Antibody User Review

    Caspase-8 Antibody for Western Blot

    July 08, 2022
    I seeded the epithelial cells for 24 hours. I starved the cells for 4 hours (0% FBS) and I induced for 24 hours with PDFG and ... read more
  • Antibody User Review

    Great Cyclin D1 Antibody for WB

    July 08, 2022
    I seeded MCF-7 (invasive ductal carcinoma) with complete medium 10% FBS; then I stimulated the cells for 24 hours in starvation ... read more
  • Antibody User Review

    Great pAMPKa Antibody for Western Blot

    June 14, 2022
    I seed the cells and I wait 24 hours leaving them at 37 °C, 5% CO2. I stimulated the cells for 24 hours and I harvested with lysis... read more
  • Antibody User Review

    Good TFAM Antibody for Western Blot

    June 14, 2022
    Cells were seeded and left overnight at 37 °C and 5% CO2. The day after were stimulated for 24 hours with 0.5% FBS. I harvested ... read more
  • Antibody User Review

    Great PDGF Used for Western Blot Detection

    June 08, 2022
    I seeded the epithelial cells for 24 hours. I starved the cells for 4 hours (0% FBS) and I induced for 24 hours with PDFG and ... read more
  • Antibody User Review

    Great Bcl-xL Detection With WB

    June 08, 2022
    I treated the epithelial cells with eiF4E siRNA for 24 hours. The knockdown of eIF4E suppresses cell growth and correlates with ... read more
  • Antibody User Review

    Great NCAM-1 for Western Blot

    June 08, 2022
    Human pluripotent stem cells (hPSCs) were cultured in a pluripotent maintenance medium. NCAM1 (a known neural-restricted protein ... read more
  • Antibody User Review

    Caspase-3 Western Blot Detection

    June 07, 2022
    I used this antibody on epithelial cells starved for 4 hours (0% FBS) and treated with eIF4E siRNA for 24 hours with F-12k medium ... read more
  • Antibody User Review

    pAKT Western Blot Detection

    June 07, 2022
    I used this antibody on epithelial cells starved for 4 hours (0%FBS) and treated with eIF4E siRNA for 24 hours with F-12k medium (... read more
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