Great NCAM-1 for Western Blot

NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

Human/Mouse NCAM-1/CD56 Antibody

Catalog Number:

AF2408-SP

Clone Number:

N/A

Info:Supplier Page

Browse Similar Products: CD56 Antibodies

Human pluripotent stem cells (hPSCs) were cultured in a pluripotent maintenance medium. NCAM1 (a known neural-restricted protein involved in cell-cell interactions) was used as an indicator of endogenous cell-type-specific protein carry-over.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human pluripotent stem cells

Primary Incubation

Antibody was diluted with Milk to 0.5ug/mL

Blocking Agent

Milk

Secondary Incubation

Antibody was diluted with Milk at a ratio of 1:2000

Tertiary Incubation

Didn't use.

Detection

ChemiDOC

Results Summary

Human pluripotent stem cells (hPSCs) were cultured in a pluripotent maintenance medium. NCAM1(a known neural-restricted protein involved in cell–cell interactions) was used as an indicator of endogenous cell-type-specific protein carry-over.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Accessible and high quality.

The Bad

No bad things.

The Bottom Line

I would buy it again.

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