Great Bcl-xL Detection With WB

June 08, 2022

NanoEngineering
UC San Diego NanoEngineering Department
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling

Product Name:

Bcl-xL Antibody

Catalog Number:

2762

Clone Number:

N/A

Info:Supplier Page View Product Specs

Browse Similar Products: Bcl-xL Antibodies

I treated the epithelial cells with eiF4E siRNA for 24 hours. The knockdown of eIF4E suppresses cell growth and correlates with increase in Bax/Bcl-2 ratio. Band was detected at 30 kDa.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Epithelial cells

Primary Incubation

1:1000 overnight at 4 °C

Blocking Agent

BSA 5% in 0.1% TBST

Secondary Incubation

Rabbit RT for 2 hours

Tertiary Incubation

Didn't use.

Detection

ChemiDOC

Results Summary

I treated the epithelial cells with eiF4E siRNA for 24 hours. The knockdown of eIF4E suppresses cell growth and correlates with increase in Bax/Bcl-2 ratio. Band was detected at 30 kDa.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Accessible and good signal.

The Bad

No bad things.

The Bottom Line

Would buy it again.

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