Daniel Hamilton

Graduate Student

UC San Diego NanoEngineering Department

Great Cleaved Caspase-8 for Western Blot
June 07, 2022
Epithelial cells were seeded. After 24 hours I used starvation medium (0% FBS) for 4 hours and I treated with eiF4E siRNA for 24 ... read more
Good Expression with AKT Antibody for Western Blot
May 26, 2022
I evaluated the total amount of AKT on A549 (epithelial lung cells) transfected for 24 hours with eIF4E siRNA. I got great results... read more
Excellent Antibody for Western Blot
May 25, 2022
I was interested in evaluating RAS pathway, especially ERK on A549. I stimulated the cells IGF-1 for 24 hours and lysed my cells ... read more
Great eIF4E Antibody for Western Blot
May 25, 2022
I seeded epithelial cells (A549) and I transfected with eIF4E siRNA after 24 hours. I seeded 2 different seeding densities (180k ... read more
Good SirT3 Antibody from Cell Signaling
May 24, 2022
I seed epithelial cells (lung cancer cells) for 24 hours and I treated with eIF4E siRNA for 24 hours. I evaluated SirT3 expression... read more
Reliable Glucose Assay Kit
May 24, 2022
I used this to evaluate the glucose consumption on my cells stimulated with TGF-b. I followed the passage as written in the kit ... read more
Good 4E-BP1 Antibody for Western Blot
May 24, 2022
I stimulated epithelial cells for 24 hours with TGF-b and I transfected eIF4 siRNA for 24 hours with starvation medium (0.5% FBS).... read more
Good Phospho-Stat3 Antibody for Western Blot
May 23, 2022
I seeded epithelial cells with 10% FBS. The day after, I replaced the medium (starvation medium, 0.5%) and treated it with eIF4E ... read more
Good Phospho-mTOR Antibody with Optimization
May 23, 2022
I seeded epithelial cells with 10% FBS. The day after I replaced the medium (starvation medium, 0.5%) and I treated with eIF4E ... read more
Great HIF-1a Antibody for Western Blot
May 23, 2022
I exposed my endothelial cells for 24 hours in hypoxia chamber (O = 1% and CO2 = 5%). I diluted the primary antibody up to 1:1000 ... read more
Good Secondary Antibody for Western Blot
May 20, 2022
I used this antibody several times and I always got great results. I diluted 1:10000 with 5% BSA in TBST and I incubated my ... read more

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Effective and Easy-to-Prepare Reagent for Cell Permeabilization

Cell membrane permeabilization for intracellular staining and related cellular assays. I have used saponin as a permeabilization reagent when preparing cells ... read more

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