Fig 2: ß-cell Glut2 knockdown (ß-Glut2 KD) mice exhibit normal glucose tolerance. Results obtained from oral glucose tolerance tests in male, n = 9 or 12 (A,B) and female, n = 6–10 (C,D) mice 4 (A,C) and 8 (B,D) weeks after inducing ß-cell Glut2 deficiency. Area under the curve (AUC) is shown in inset bar graphs. The mice were 8–10 weeks old when ß-cell Glut2 deficiency was induced. Immunofluorescence, n = 6 (E) and qRT-PCR, n = 6 (F) demonstrating upregulation of islet Glut1 in female ß-Glut2 KD mice after completion of the study. 3 islets per section and 3 sections per mouse were quantified (E). Ins +ve and -ve, quantification of GLUT1 fluorescence in insulin positive and negative staining. Scale bar is 50 µm. Control, Glut2loxP/loxP mice; AU, arbitrary unit. Student’s unpaired t-test was used for comparisons, * p < 0.05, ** p < 0.01, *** p < 0.001. Error bars are mean ± SEM.