Cell Signaling
PDGF Receptor β (28E1) Rabbit mAb
3169
N/A
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I seeded the epithelial cells for 24 hours. I starved the cells for 4 hours (0% FBS) and I induced for 24 hours with PDFG and treated them simultaneously with eiF4E siRNA in 2% FBS. I got a clear band. I suggest using low voltage during the western due to the high molecular weight of the protein.
Western Blot
Epithelial cells
1:1000 overnight at 4 °C
BSA 5% in 0.1% TBST
Rabbit RT for 2 hours
Didn't use
ChemiDOC
I seeded the epithelial cells for 24 hours. I starved the cells for 4 hours (0% FBS) and I induced for 24 hours with PDFG and treated simultaneously with eiF4E siRNA in 2%FBS. I got a clear band. I suggest to use low voltage during the western due the high molecular weight of the protein.
Accessible and high quality.
No bad things.
Not really expensive and great results.