Anti-epidermal growth factor antibodies are used for the immunodetection of the protein encoded by the EGF gene. In humans, the canonical protein has a reported length of 1207 amino acid residues and a mass of 134 kDa. Its subcellular localization is in the membrane. Alternative splicing is reported to yield 3 different isoforms for this protein. It is reported to be expressed in the kidney, salivary gland, cerebrum and prostate. It is noted to be involved in angiogenesis. Post-translational modifications have been described, including O-glycosylation. The gene encoding this protein is implicated in Hypomagnesemia. Other names for this target antigen include URG, pro-epidermal growth factor, beta-urogastrone, and HOMG4. Gene orthologs have been identified in the mouse, rat, bovine, frog, zebrafish, chimpanzee and chicken species. Over 140 citations in the literature describe the use of epidermal growth factor antibodies in research. Western Blot is a widely used application for these antibodies. ELISA and Immunohistochemistry are also common applications.