Santa Cruz
EGFR (E-8) antibody
sc-374607
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Browse Similar Products: EGFR Western Blot Antibodies
Mouse mAb sc-374607 against EGFR from Santa Cruz was used in western blotting for detection of EGFR in the lysates of human tumor cells. The antibody produced detected EGFR in a proper region of ~180 kDa, but the protein was detected as a double-band.
Western Blot
Tumor cell lysates
Overnight, at 4-8 degrees Celsius, with rocking, at 1 ug/mL in 5% milk/PBS/0.05% Tween 20
5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw)
Goat anti-Mouse IgG HRP-conjugated antibody # 7076 from Cell Siganaling; 1:3,000
None
SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific)
The mouse mAb sc-374607 produced overall good results, but the EGFR protein was detected as a double band, which did not happen with other anti-EGFR antibodies tested in parallel.
The antibody appeared specific since no additional bands were detected through the whole length of the blot.
The antibody detected EGFR in the expected region of ~180 kD.
EGFR was detected as a double band.
There are better anti-EGFR antibodies on the market, including the antibodies from Santa Cruz