Cell Signaling Technology
Phospho-EGF Receptor (Tyr1068) (D7A5) XP® Rabbit mAb
3777
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This antibody was used in a study to examine phosphorylation and signaling of EGF Receptor. U251 human glioma cells were serum starved overnight, incubated in 10ug/ml Cycloheximide to block new protein synthesis and then treated with EGF ligand to stimulate receptor internalization and phosphorylation.
Western Blot
U251 human glioma cell lysate
1:1000
5% Milk in TBS- 0.2% Tween
Goat anti-rabbit antibody; 1/3000
None
Femto HRP Chemiluminescent substrate Kit
Antibody works very well! Antibody identifies a specific band. Result shows phosphorylation of EGF Receptor by 30 minutes of EGF ligand stimulation and then a gradual decline in receptor phosphorylation. This is consistent with what is known about the EGF Receptor phosphorylation events in most cell types
Antibody works well
Antibody works very well