Cell Signaling
Phospho-EGF Receptor (Tyr1068) Rabbit Antibody
2234
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Functional activity of epidermal growth factor receptor (EGFR) is associated with the autophosphorylation of several tyrosine (Y) residues in the C-terminal domain. Detection of phosphorylated EGFR is important in cell biology, especially in studying cancer cells. We used Cell Signaling rabbit antibody 2234 that detects phosphorylation at Y1068 to determine the levels of EGFR activation and the efficiency of EGFR inhibitors in preventing EGFR phosphorylation.
Western Blot
Lysates of human tumor cells; 20 microgram per lane
Antibody at a final concentration of 1 microgram/ml, ON incubation with gentle rocking in a cold room
5% BSA in PBS-0.05% Tween; 45-60 min, RT, gentle rocking
Goat anti rabbit IgG conjugated with HRP, 1:3,000; 1 hr; RT; gentle rocking
N/A
West Pico
Rabbit antibody 2234 produces a single protein band of approximately 180 kDa, which corresponds to expected mol. mass of EGFR.
The image demonstrates that in the presence of an EGFR inhibitor, Erlotinib, detection of phosphorylated EGFR (pEGFR) is significantly reduced, indicating that Erlotinib efficiently inhibited EGFR activation. Similar levels of total EGFR in the same samples confirm equal loading of EGFR protein.
The antibody appears highly specific and sensitive.
None detected.
The rabbit antibody 2234 from Cell Signaling is highly recommended for detection by western blotting of phosphorylated EGFR in lysates of human tumor cells.