Very Good EGFR Antibody

Molecular Oncology
Moffitt Cancer Institute
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

EGF Receptor (D38B1) XP® Rabbit mAb

Catalog Number:

#4267

This antibody was selected as part of the ImmunoOncology project where we performed western blot and IHC for EGFR on Human Lung Cancer Tissues. The antibody is very specific and the results were in corroboration with another analysis. Works very well in paraffin sections, never had any issues.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Human Lung Cancer Tissues

Primary Incubation

12 hours

Blocking Agent

H2O2

Secondary Incubation

2 hours

Tertiary Incubation

30 mins

Detection

80%

Results Summary

Works very well for IHC

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Very specific

The Bad

needs in higher concentration

The Bottom Line

Can be used for IHC and IP

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