Cell Signaling Technologies
EGF Receptor
2085S
D6B6
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EGFR is a receptor for epithelial growth factor that initiates cell proliferation, migration and invasion in cancer cells. I tested this antibody for determining the expression of EGFR in human cancer cell lines, HeLa, MCF7 and SiHa.
Western Blot
Human cancer cell lines: Hela, MCF7 and SiHa
1:1000 dilution, 18 hours at 4 degrees with gentle shaking
5% non-fat dry milk in Tris buffer containing 0.1% Tween-20
HRP-conjugated anti-rabbit antibody 1:2000 dilution, 1 hour at room temperature with gentle shaking
Not applicable
ECL based detection.
EGFR is abundantly expressed in most cancer cell lines including HeLa, MCF7 and SiHa. This antibody worked great for detection of EGFR protein by Western Blotting.
Specificity
Nothing
Overall, its a great antibody for Western blot based detection of EGFR protein.