Santa Cruz
EGFR (C-2) antibody
sc-377229
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Browse Similar Products: EGFR Western Blot Antibodies
The mouse mAb sc-377229 from Santa Cruz was used to detect EGFR in the lysates of human tumor cells. The antibody produced excellent results since only one protein band with the mol. wt. of ~180 kDa, consistent with the size of EGFR, was identified.
Western Blot
Tumor cell lysate
Overnight, at 4-8 degrees Celsius, with rocking; at 1 ug/mL in 5% milk/PBS/0.05% Tween 20
5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw)
Goat anti-Mouse IgG HRP-conjugated antibody # 7076 from Cell Siganaling; 1:3,000
None
SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific)
The mAb sc-377229 from Santa Cruz nicely detected EGFR in the lysates of human tumor cells. Only one band with mol. wt. of ~180 kda, consistent with the size of EGFR, was detected for each of 3 different cell lines tested.
The antibody appears to be highly sensitive and specific (did not produce any non-specific bands).
None detected
I would highly recommend this antibody for detection of EGFR in human tumor cells by western blotting.