Anti-CD86 antibodies are essential reagents in flow cytometry for detecting the target protein, CD86 molecule. CD86 binds to the costimulatory molecule CD28 on naïve T cells, promoting T cell responses, and to the inhibitory receptor CTLA-4, inhibiting T cell responses. This interaction is essential for T-lymphocyte proliferation and interleukin-2 production. CD86 also regulates B cell function, influencing the level of IgG1 produced. It localizes to the cell membrane. CD86 has been used as a phenotypic marker for microglia, endothelial cells, monocytes, macrophages, B cells, neutrophils, and eosinophils. Examine the supplier's provided validation data when comparing CD86 antibodies for flow cytometry. Also look for application-specific recommendations, such as dilutions, protocols, sample types, and other helpful usage notes.