Antibody Works Great for Staining CD86

Stanford

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 785™ anti-mouse CD86 Antibody

Catalog Number:

105043

Clone Number:

GL-1

Info:Supplier Page View Product Specs

Browse Similar Products: CD86 Antibodies

We wanted to assess how certain perturbations would affect the activation state of immune cells in the spleen, so we used the antibody to identify CD86+ immune cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse spleen

Primary Incubation

1:100

Blocking Agent

Fc Block

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

Attune NxT cytometer

Results Summary

We stained mouse spleen cells with the anti-CD86 antibody and found that it identifies CD86+ immune cells very well. The antibody was easy to use and the staining was very clear.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

The antibody works great for staining CD86.

The Bad

None to mention.

The Bottom Line

The antibody stains CD86 well, allowing good examination of expression.

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