Great PE/Cyanine5 anti-human CD86 Antibody from BioLegend

February 02, 2023

AG 61
Leibniz Institute für Virologie
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

CD86 anti-human PE-Cy5

Catalog Number:

305408

Clone Number:

IT2.2

Info:Supplier Page View Product Specs

Browse Similar Products: CD86 Antibodies

The antibody works perfectly on PBMCs. Bright signal and good separation between positive and negative populations.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PBMCs

Primary Incubation

1µl/sample, 20 minutes,RT

Blocking Agent

NHS

Secondary Incubation

No secondary incubation needed.

Tertiary Incubation

No tertiary incubation needed.

Detection

PE-Cy5

Results Summary

Working greatly with cells isolated from blood. Good positive pick and brightness. The concentration required to be used on human PBMCs is 1µl/sample. It is working perfectly extracellularly and it doesn´t lose the signal also after fixation.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Brightness and affordable.

The Bad

Nothing bad.

The Bottom Line

Highly recommended antibody for FACS on human PBMCs.

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