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FITC anti-mouse CD86 Antibody
105005
GL-1
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I used this antibody to characterize the non-adherent cells that were isolated from murine derived bone marrow cell culture. Cells were cultured in the presence of GM-CSF. CD86 is one of the dendritic cell maturation markers.
Flow Cytometry
Mouse bone marrow derived dendritic cells
1:100 at 4 degrees for 30 minutes
2% FBS in PBS
Not applicable
Flow cytometry
CD86 was detected on dendritic cells differentiated from mouse bone marrow cells ex vivo.
N/A
High specificity
Nothing
This antibody performed well in the flow cytometry-based assay.