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CD86 BV650
105036
GL1
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We use single cell analysis methods (flow cytometry, scRNAseq, etc) to study the mechanisms responsible for immune tolerance.
Flow Cytometry
Mouse Lymph nodes, spleen
Block: 1:50 in 100uL at 37C for 30 min
Fc Block (aCD16/32)
L/D: Zombie NIR 1:4000 in 100 uL at RT for 20 min
Surface stain: 1:200 in 100uL at 4C for 30 min
Spectral flow cytometry (Cytek Aurora)
Good staining for costimulatory molecules on antigen presenting cells. Fairly reliable results that enable reasonably clean separation of + from - APCs when looking at DCs, macrophages, etc individually, although separate optimization is needed for different instruments to get it to work well.
Cheap and good signal
Can have somewhat variable reliability across different instruments.
Great, affordable antibody for studying antigen presenting cells.