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Brilliant Violet 421™ anti-human CD86 Antibody
305425
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A CD86 antibody that is more sensitive than the competitor's analogue. Accurate, good staining for human monocytes and macrophages (or stem cell-derived monocytes and macrophages).
Flow Cytometry
Human monocytes and macrophages
Dilution used was 1:45 for 30 minutes on ice
FACs buffer (1.5% FBS in PBS)
Not required as its a conjugated antibody for FACs
Not required
Flow cytometry
Excellent antibody providing very good staining.
N/A
Good staining in human cells
Titration curve needs to be performed first to optimize the dilution
Would definitely recommend this antibody for staining of human primary cells of the monocytic/macrophage lineage.