Staining CD86 in Skin Cells

December 20, 2019

BlueskyReddit
UIC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Thermo Fisher Scientific

Product Name:

CD86 (B7-2) Monoclonal Antibody (GL1), PE-Cyanine7

Catalog Number:

25-0862-82

Clone Number:

GL1

The was to detect CD86 positive cells in skin wounds. The antibody worked well.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse skin wounds

Primary Incubation

0.25ug for one million cells in 100ul buffer

Blocking Agent

FC blocker

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

This antibody had strong detection of CD86 cells in skin wound cells. Easy to use.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Easy to use, strong signal

The Bad

None

The Bottom Line

Excellent antibody to detect mouse CD86 cells.

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