Flow Cytometry Antibody Products

Excellent PE Isotype Mouse IgG2b

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BioLegend
This antibody was used as an isotype control in intracellular flow cytometry and compared to anti-HMGB1-PE to determine the specifity of the HMGB-1 antibody.
FITC-Conjugated Monoclonal Mouse Anti-Human CD3 Antibody (for Flow Cytometry) from BioLegend (Clone UCHT1)

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BioLegend
The monoclonal mouse anti-human CD3 antibody, clone UCHT1, is one of the more established monoclonals used for CD3 staining of T cells. We routinely use this antibody to identify the CD3+ T cell ... Read Review
A Very Good CD8 Antibody for Flow Cytometry from BioLegend: PE-Conjugated Monoclonal Mouse Anti-Human CD8 alpha

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BioLegend
The general aim of our research is to further identify and understand the role that minor populations of lymphocytes have in immunity. To first identify these lymphocyte subpopulations, we use flow ... Read Review
CD3, CD4, CD8 Antibodies

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BioLegend
These antibodies from BioLegend work extremely well. I used these to phenotype in vitro expanded primary T cells. These antibodies give me consistent results every time. Although the CoA says use 5 ul... Read Review
Great F4/80 Antibody

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BioLegend
I use this antibody for labeling Kupffer cells from murine livers. On a BD LSRII, BD Aria IIu, or a BD Canto II, I see my cell population shift to the 4th decade with minimal background when using the... Read Review
Brilliant fluorochrome for a dim marker

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BioLegend
This antibody was used in a staining panel to identify Tregs in the thymus and the spleen. The CD25-Brilliant Violet 421 antibody had a great noise-to-signal ratio and also withstood the fixation and ... Read Review
Excellent recognition of CD68+ macrophages in various tissues of the mice

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Bio-Rad (Formerly AbD Serotec)
Macrophages can be identified in tissue cross-sections via the CD68 intracellular marker. We used this product along with the use of Cell Signaling Tech's AlexaFluor488 (Catalog No. 4416S) secondary ... Read Review
Anti-HA (16B12) Biolegend

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BioLegend
U2OS cells transfected with HA-SceI were lysed in sample buffer and proteins were separated in 4-20% gradient SDS-PAGE. Ha-SceI expression was probed by anti-HA (16B12) (1:1000) from BioLegend. The ... Read Review
Labeling CD16 Positive Cells in Human PBMC Sample

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BioLegend
This is a good antibody for discriminating CD16 postive and negative cells such as monocytes, etc
Anti-Mouse Gr1 BV421 for Splenocyte Staining

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BioLegend
This product was used to identify Grf+ cells amongst splenocytes (eg neutrophils or monocytes) under different conditions (eg tumor bearing vs non tumor bearing mice).
Mouse Skin Neutrophils

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BD Biosciences
Our goal is to detect neutrophils in inflamed mouse skin using IF staining. This antibody is specific, easy to use, less expensive, and the result is beautiful.
Good Antibody to Identify HLADR+ Immune Cells

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BioLegend
The aim of this study was a phenotypic analysis of human peripheral blood using multicolor flow cytometry.
Trusted CD11c Antibody

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BD Biosciences
I have used this item for years for my research. It is consistent between lots and offers strong staining.
Good Marker for Peritoneal Macrophages

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BioLegend
We are interested in studying macrophage activation phenotypes in mice. We collect peritoneal exudate cells, and use flow cytometry to gate out the non-macrophage population. This antibody provides ... Read Review
Good General Purpose B Cell Marker

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BioLegend
We have an immunology lab that studies the phenotypes of B cells in mice. This antibody is very effective at labeling B cells from total splenocyte preparations.
Good Marker for Sorting Naive CD4 T Cells

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BioLegend
We study T cell responses to antigen presenting cells. This is an excellent antibody for gating populations of CD4+ T cells in murine spleens.
Very Good Anti-Human CD8 antibody

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BioLegend
I have used this antibody to analyze the fraction of CD8+ T cells isolated from splenocytes of humanized mice. The staining looks good with two separate populations of CD8 positive and negative cells.
Donkey Anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 647 Conjugate

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Thermo Fisher Scientific
This is a great product to do immunofluorescence. We study primary cells (airway epithelial cells) in our lab and can use several different donkey alexas with different fluorophore conjugations in a ... Read Review
Good and useful reagent

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BioLegend
The aim of this research is to study bone marrow progenitor cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell counts. We selected CD19 Antibody from... Read Review
Very Useful CD4 Antibody

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BioLegend
The aim of my research is to study lymph node cells in cancer. In my experiment, I tested if a cytokine associated with tumor promotion alters cell counts. We selected CD4 Antibody from Biolegend ... Read Review

Flow Cytometry Antibody Products

Flow Cytometry Antibody Products

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Invitrogen Antibodies

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Leinco Technologies, Inc.

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BioLegend

Invitrogen Antibodies

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Bio X Cell

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Proteintech Group Inc

MyBioSource.com

Tonbo Biosciences

Leinco Technologies, Inc.

Biogems International, Inc.

Scicons

Flow Cytometry Antibody Products

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