Human/Mouse CD117/c-kit Antibody AF1356 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemHuman/Mouse CD117/c-kit Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $519.00Supplier Page View Company Product Page
Catalog NumberAF1356
NCBI Gene AliasesPBT, Fdc, MASTC, C-Kit, Gsfsow3, SOW3, W, CD117, SCFR, Ssm, SCO5, SCO1, Bs, Gsfsco1, Tr-kit, Gsfsco5, c-KIT
IsotypeIgG
ApplicationsFlow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,Simple Western,Neutralization,Immunofluorescence,Immunocytochemistry,CyTOF-ready,Dual RNAscope ISH-IHC Compatible,Immunocytochemistry/ Immunofluorescence
HostGoat
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
TargetCD117/c-kit
Conjugate/TagUnconjugated
Quantity100 ug (also 25 ug)
ReactivityHuman,Mouse,Porcine,Rat,Transgenic Mouse
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse CD117/c-kit, Gln25-Thr519 (Ala207Glu), Accession # P05532
ConcentrationLYOPH
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution
PurityAntigen Affinity-purified
ClonalityPolyclonal
NCBI Full Gene NameKIT proto-oncogene receptor tyrosine kinase, KIT proto-oncogene, receptor tyrosine kinase

Specifications/Features

Available conjugates: Unconjugated

Questions and Answers

Can this antibody be used to study c-Kit expression in mouse models, particularly in relation to spermatogenesis?

Yes, the CD117/c-kit antibody has been utilized in studies examining spermatogenesis in mouse models. For instance, it was used to identify c-Kit expression in mouse testis explants, highlighting its role in the meiotic process and the implications of temperature sensitivity in spermatogenesis (Hirano et al., 2022).

Is the CD117/c-kit antibody effective for immunofluorescence applications in detecting c-Kit in various tissues?

The CD117/c-kit antibody has been shown to work effectively in immunofluorescence applications. It was employed in studies to visualize c-Kit expression in different contexts, including its role in cardiac progenitor cells and interstitial cells of Cajal in the gastrointestinal tract (Van Berlo et al., 2014; Wang et al., 2022).

What species can this antibody detect, and is it suitable for studies involving human tissues?

The CD117/c-kit antibody is reactive with multiple species, including humans, mice, rats, and porcine tissues. This broad reactivity makes it suitable for studies involving human tissues as well as various animal models (R&D Systems).

Are there any specific applications in cancer research where this antibody has been cited?

Yes, the CD117/c-kit antibody has been cited in cancer research, particularly in studies investigating the role of c-Kit in tumor angiogenesis and progression. For example, it was used to assess c-Kit expression in the context of tumor vascularization and immune response modulation (Wagner et al., 2014).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) 3D imaging with enhanced transparency, signal-to-background ratios, and antigen detection using HyPer-3DCell Reports MethodsApril 20, 2026Tansol Choi, Lauretta A. Lacko, Neranjan de Silva, Nang Kham Hsi Liang, Ying Liu, Edgar A. Jamies, Todd Evans, Romulo Hurtado1:500), anti-GFRα1 (AF560, R&D Systems at 1:200), anti-STRA8 (Ab49405, Abcam at 1:200), and anti-cKIT (AF1356, R&D Systems at 1:200). Minimally cross-absorbed Alexa Flour secondary antibodies were used to detect

(2) RUNX proteins and CBFβ create an interconnected network of transcriptional regulation in the male germline†Biology of ReproductionApril 13, 2026Mustika Rahmawati, Carson J Black, Danika D Dalvi, Amanda J Brown, Kassie M Stadler, Tia M Hoisington, Nathan C Lawanti-LIN28A,"R&D Systems, AF3757","IF, Flow cytometry",IF 1:250Flow cytometry 1:125 Goat anti-cKIT,"R&D Systems, AF1356",IF,1:125 Goat anti-SOX3,"R&D System, AF2569",IF,1:250 Goat anti-GFR⍺1,Fisher - AF560,IF,1:250 Rat

(3) Undifferentiated spermatogonia modulate their behavior via the expression of basement membrane protein laminin†Biology of ReproductionApril 13, 2026Yusuke Kawabe, Saya Yamada, Yuichi Shima, Kentaro Tanemura, Shosei Yoshida, Kenshiro Haraanti-PH3 (1:500 dilution; Merck, Darmstadt, Germany: 06-570), anti-cKit (1:1000 dilution; R&D Systems: AF1356), anti-Laminin gamma 1 clone A5 (1:50 dilution; Sigma Aldrich, Burlington, MA, USA: MAB1914P), and anti-GFP

(4) Novel imaging approach for simultaneous tracking of cell dynamics in distinct tissue layers reveals cells involved in colonic peristalsisFrontiers in ImagingJanuary 28, 2026Salah A. Baker, Peter J. Blair, Sharif Amit Kamran, Kenton M. Sanders48 h at 4°C. The primary antibody used in this study was a polyclonal raised against c-Kit (mSCFR, AF1356, R and D Systems, MN, USA; 1:100 dilution in 0.5% Triton-X working solution). Next, the tissues were

(5) Ex Vivo Spatiotemporal Characterization of Spermatogenesis in Mouse Testicular OrganoidsAdvanced ScienceJanuary 1, 2026Jiachen Sun, Lifa Zhu, Yutong Li, Jinyan Tang, Tao Zhang, Hengjia Zhang, Wanchu Wang, Lufan Li, Shuhui Bian, Xin Wuab13840), PLZF (1:400, R&D Systems, AF2944), STRA8 (1:200, Abcam, ab308124), c‐KIT (1:200, R&D Systems, AF1356), SYCP3 (1:400, Abcam, ab97672), γH2AX (1:500, Abcam, ab11174), H1t (1:500, ABclonal, A18597), CREM

(6) A comprehensive study of c-Kit-expressing amacrine cells in the mammalian retinaSci RepDecember 5, 2025Zheng Jiang, Wen ShenPrimary antibody,Dilution,Source (Cat#) Goat anti-CD117,1:500,R&D system (AF1356) Rabbit anti-GABA,1:1000,Millipore (AB175) Rabbit anti-disable-1,1:500,Sigma (HPA052033) Goat anti-ChAT,1:1000,Millipore

(7) Enhanced Spatial Mapping of Mouse Gastric Muscle Layers Using a Modified Swiss Roll TechniqueJournal of Visualized ExperimentsNovember 25, 2025Nick M. Huynh, Srinidhi R. Babu, Egan L. Choi, Tamas Ordog, Yujiro HayashiScissors,FINE SCIENCE TOOLS,14090-09, Hematoxylin,Epredia,7211, KIT (0.2 μg/mL; 1:1000 dilution),R&D Systems,AF1356,Lot: IEO0217101 RRID: AB_354750 LENS CLEANING SOLUTION 1OZ SPRAY BOTTLE,Nikon,77013066, Microscope

(8) Secretin targets interstitial cells of Cajal to regulate intestinal contractionsEMBO ReportsNovember 6, 2025Allison M Bartlett, Peter J Blair, Kenton M Sanders, Salah A BakerH-1800 Antibodies,Antibodies,Antibodies Goat polyclonal primary antibody- mSCFR,R and D systems,Cat. No. AF1356 Donkey anti-goat Alexa Fluor® 488,Jackson ImmunoResearch Laboratories Inc.,Cat. No. 705-545-147 Reagents

(9) A comprehensive molecular atlas of the mesenchymal cell types in the mouse liverEMBO ReportsSeptember 15, 2025Riikka Pietilä, Guillem Genové, Giuseppe Mocci, Yuyang Miao, Jianping Liu, Stefanos Leptidis, Francesca Del Gaudio, Martin Uhrbom, Elisa Vázquez-Liébanas, Sonja Gustafsson, et al.(rat),eBiosciences,14-5851-82 EPCAM-APC (rat),eBiosciences,17-5791-82 GPMA6A (rat),LS-Bio,LS-C179305 KIT (goat),R&D Systems,AF1356 LYVE1 (rabbit),Cell Signaling Technology,67538S NCAM1 (goat),R&D Systems,AF2408 NGFR (rabbit),Abcam,ab52987 NPNT

(10) c-Kit signaling confers damage-resistance to sweet taste cells upon nerve injuryInt J Oral SciJuly 29, 2025Su Young Ki, Jea Hwa Jang, Dong-Hoon Kim, Yong Taek Jeongpaper54), goat anti-Car4 (1:500, AF2414, R&D Systems, Minneapolis, MN, US), goat anti-c-Kit (1:400, AF1356, R&D Systems), rabbit anti-GNAT3 (1:1 000, sc-395, Santa Cruz, Dallas, TX, US), goat anti-GNAT3 (1:1 000

(11) A Novel Cultivation System for Germ Cell Proliferation and Sustaining Whole Testicular NicheAdvanced ScienceJuly 28, 2025Yu Xia, Xiaoxuan Zhang, Bohang Zhang, Minhui Lu, Qing Cheng, Li Liu, Lifang Shi, Yiqiang Cui, Dingdong Chen, Laihua Li, et al.617300), GFRα1 (1:500; R&D systems, AF560), PLZF (1:500; R&D systems, AF2944), CKIT (1:200; R&D systems, AF1356), KI67 (1:500, Abcam, Ab16667), SYCP3 (1:200, Abcam, ab97672). The following secondary antibodies were

(12) Unraveling the development of cutaneous neurofibromas in neurofibromatosis type 1Acta Neuropathol CommunJuly 19, 2025Pernelle Pulh, Fanny Coulpier, Audrey Onfroy, Layna Oubrou, Wanzhen Zhang, Léa Toledano, Elie Abou Zougheib, Laura Fertitta, Pierre Wolkenstein, Piotr TopilkoAbcam #ab15580), rabbit anti-P-ERK (1:250; Cell Signaling #9101), goat anti-cKIT (1:100; R&D system #AF1356), rat anti-F4/80 (1:400; Abcam #ab6640), rat anti-MBP (1:200; Chemicon Millipore #MAB386), goat anti-POSTN

(13) Biomimetic Catechol-incorporated Polyacrylonitrile Nanofiber Scaffolds for Tissue Engineering of Functional Salivary GlandsBiomaterials ResearchJuly 2, 2025Seokjun Kwon, Ji Hyun Ryu, Junchul Kim, Hyun Ho Shin, Gehoon Chung, Ali Taghizadeh, Jung-Hwan Lee, Jongho Kim, Bon-Cheol Ku, Kyungpyo Park, et al.used for immunofluorescence staining are as follows: oat polyclonal anti-c-kit antibody (R&D Systems, AF1356), beta-III tubulin antibody (TUJ1; R&D Systems, MAB1195), Ki67 monoclonal antibody (Invitrogen, 14-5698-82),

(14) Commitment of adipose-resident c-kit(+) progenitors to brown adipocytes contributes to adipose tissue homeostasis and remodelingNature communicationsJuly 1, 2025Chen Q, Yu Y, Zhang R, Zhao Q, Yu D, Feng C, Zhou J, Luo M, Yang M, Sun S, et al.(Abcam, ab61682, 1:500), anti-UCP1 (Novus Biologicals, NB100-2828, 1:200), and anti-c-kit (R&D Systems, AF1356, 1:100). Alexa Fluor 488- or 555-conjugated secondary antibodies (Invitrogen, 1:1000) were used to detect

(15) Enhanced neoangiogenesis and balance of the immune response mediated by the Wilms' tumor suppressor WT1 favor repair after myocardial infarctionTheranosticsJune 9, 2025Nicole Wagner, Ana Vukolic, Delphine Baudouy, Sophie Pagnotta, Jean-Francois Michiels, Kay-Dietrich WagnerCD45 (1:50, rat monoclonal, BD Biosciences, Le Pont de Claix, France), CD117 (1:200, goat polyclonal, AF1356, R&D Systems, Lille, France), CD3 (1:100, rabbit monoclonal, clone SP7, ab16669, Abcam), GR-1 (1:150,

(16) Engineered BMP2/BMP7 extracellular vesicles induce autocrine BMP release driving SMAD phosphorylation to promote bone formationNPJ Regen MedJune 3, 2025Zeji Du, Skylar A. Rizzo, Tiffany L. Sarrafian, Monique S. Bagwell, Ryan C. Mahlberg, Ashley Amontree, Paige Schiebel, Dinah M. Tauferner, Zoe S. LeBrasseur, Tyra A. Witt, et al.using either α-smooth muscle actin primary antibody (1:1000, Abcam, #ab7817), CD117 (1:50, Novus, #AF1356), and CD34 (1:100, R&D, #AF4117) with hematoxylin counter stain. All slides were scanned, and images

(17) Functional roles of interstitial cells of Cajal in the GI tract of ratsAmerican Journal of Physiology-Gastrointestinal and Liver PhysiologyJune 1, 2025Sung Jin Hwang, Joong Goo Kwon, Elizabeth A. H. Beckett, Minkyung Kim, Tom Herbert, Kenton M. Sanders, Sean M. WardTarget,Resource,Cat No. RRID No.,Clone,Host,Dilution,Fixative c-KIT,"R&D Systems Inc., Minneapolis, MN, USA",AF1356,Poly-,Goat,1:100,Zamboni’s ,,,,,, ANO1,"Abcam, Cambridge, MA, USA",AB323181,Poly-,Rabbit,1:800,Zamboni’s ,,,,,, vAChT,"Millipore

(18) BRCA1 preserves genome integrity during the formation of undifferentiated spermatogoniaEMBO ReportsMay 28, 2025Peng Li, Licun Song, Longfei Ma, Chunsheng Han, Lejun Li, Lin-Yu Lu, Yidan Liu2009",N/A Rabbit anti-PLZF,Santa Cruz,sc22839 Goat anti-PLZF,R&D Systems,AF2944 Goat anti-KIT,R&D Systems,AF1356 Goat anti-GFRα1,R&D Systems,AF560 Goat anti-SOX3,R&D Systems,AF2569 Rabbit anti-cleaved PARP1 (Asp214),"Cell Signaling Technology

(19) Inactivation of Histone Chaperone HIRA Unmasks a Link Between Normal Embryonic Development of Melanoblasts and Maintenance of Adult Melanocyte Stem CellsAging CellMay 14, 2025Farah Jaber‐Hijazi, Rouven Arnold, Karthic Swaminathan, Kathryn Gilroy, Alexander T. Wenzel, Anthony Lagnado, Kristina Kirschner, Neil Robertson, Claire Reid, Neil Fullarton, et al.ON at 4°C in primary antibodies: DCT (goat, Santa Cruz sc‐10,451, 1:200); c‐Kit (goat, R&D systems AF1356, 1:400); BIII tubulin (Tuj1, mouse, Promega G7121, 1:1000). After 3 × 5 min washes in PBST, cells were

(20) A specialized population of intramuscular interstitial cells of Cajal regulates motility within the mouse oesophagogastric junctionThe Journal of PhysiologyMay 5, 2025Emer P. Ni Bhraonain, Karen I. Hannigan, Jack A. Turner, Grace A. Wildbore, Kenton M. Sanders, Caroline A. CobinePBS before blocking again and incubating in the second primary antibody (anti-Kit; h/m CD117/c-kit, AF1356, R&D Systems, Minneapolis, MN, USA; RRID: AB_354750;1:100 dilution in 0.5% Triton-X 100 or anti-smMHC;

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(1)

3/1/2022Good CD117 Antibody for Immunostaining James CarrollStem cell factor receptor (CD117) and its ligand (also named c-kit ligand) play essential roles in gametogenesis, melanogenesis and hematopoiesis.

CD117/c-kit Antibody

CD117/c-kit Antibody

CD117/c-kit Antibody

CD117/c-kit Antibody

CD117/c-kit Antibody

Human/Mouse CD117/c-kit Antibody from R&D Systems, a Bio-Techne Brand

Specifications/Features

Questions and Answers

R&D Systems, a Bio-Techne Brand

Citations (144)

Reviews

Human/Mouse CD117/c-kit Antibody from R&D Systems, a Bio-Techne Brand

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