α Tubulin (B-7) Antibody sc-5286 from Santa Cruz Biotechnology, Inc.

Product Specs
Itemα Tubulin (B-7) Antibody
CompanySanta Cruz Biotechnology, Inc.
Price Pricing Info Supplier Page View Company Product Page
Catalog Numbersc-5286
Antibody TypePrimary Antibodies
CloneB-7
ApplicationsWB, IP, IF, IHC(P), FCM, ELISA
Reactivitymouse, rat, human
Quantity200 µg/ml
ClonalityMonoclonal
Conjugate/TagUnconjugated
FormatPurified
Hostmouse
NCBI Gene AliasesEG434428, K-ALPHA-1, TUBA3, MACTHC2, TUBA1, LIS3, TUBA6, bcm948, Tuba3, Tuba2, Tuba1, Tuba7, Tuba6, Tuba4, KTCN9, TUBA2, bA408E5.3, M[a]3, M[a]7, M[a]6, M[a]4, TUBAL2, CDCBM8, Tuba-1, ALS22, B-ALPHA-1, H2-ALPHA
NCBI Full Gene Namepredicted gene 5620, tubulin, alpha 4A, tubulin, alpha 1A, tubulin, alpha 8, tubulin, alpha 1C, tubulin, alpha 1B, tubulin, alpha 3B, tubulin, alpha 3A, tubulin alpha 3c, tubulin alpha 3d, tubulin alpha 3e, tubulin alpha 1c, tubulin alpha 1b, tubulin alpha 1a, tubulin alpha 4a, tubulin alpha 8
Targetalpha Tubulin
IsotypeIgG2a

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Santa Cruz Biotechnology, Inc.

10410 Finnell Street

Dallas, Texas 75220

United States

Phone: 1-800-457-3801
831-457-3801

Company Profile

Website: http://www.scbt.com/

(1) Chaperone-mediated autophagy dysregulation during aging impairs hepatic fatty acid oxidation via accumulation of NCoR1Molecular metabolismOctober 1, 2023Choi YJ, Yun SH, Yu J, Mun Y, Lee W, Park CJ, Han BW, Lee BH.antibodies: NCoR1 (#5948), PKM2 (#4053) from Cell Signaling Technology; β-actin (sc-47778), α-tubulin (sc-5286), PPARα (sc-398394), and Lamin B (sc-374015) from Santa Cruz Biotechnology; LAMP2A (ab18528) from Abcam;

(2) Co-evolution of AR gene copy number and structural complexity in endocrine therapy resistant prostate cancerNAR CancerAugust 24, 2023Andrej Zivanovic, Jeffrey T Miller, Sarah A Munro, Todd P Knutson, Yingming Li, Courtney N Passow, Pijus Simonaitis, Molly Lynch, LeAnn Oseth, Shuang G Zhao, et al.antibody), AR-V9 diluted 1:1000 (generated under collaboration with RevMab), and tubulin diluted 1:4000 (sc-5286, Santa Cruz). RT-PCR Total RNA (1 μg) was used for cDNA synthesis using cDNA qScript SuperMix (Quanta

(3) Co-Targeting Nucleus Accumbens Associate 1 and NF-κB Signaling Synergistically Inhibits Melanoma GrowthBiomedicinesAugust 8, 2023Lixiang Gu, Xingcong Ren, Chrispus Ngule, Xiaofang Xiong, Jianxun Song, Zhiguo Li, Jin-Ming YangNAC1 was obtained from abcam (ab29047). An antibody against tubulin was purchased from Santa Cruz (sc-5286, Santa Cruz, CA, USA). 2.4. Western Blotting Cells were washed with PBS after harvesting and then

(4) Muscle-specific ER-associated degradation maintains postnatal muscle hypertrophy and systemic energy metabolismJCI insightAugust 3, 2023Abdon B, Liang Y, da Luz Scheffer D, Torres M, Shrestha N, Reinert RB, Lu Y, Pederson B, Bugarin-Lapuz A, Kersten S, et al.primary antibodies diluted in 2% BSA/TBST overnight at 4°C: anti-tubulin (Santa Cruz Biotechnology, sc-5286; 1:5000), anti-HSP90 (Santa Cruz Biotechnology, sc-13119; 1:5000), anti-SEL1L (made in house, ref. 20;

(5) Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in miceJHEP reports : innovation in hepatologyAugust 1, 2023Liu C, Li X, Gao M, Dong Y, Chen Z.(11029-1-AP, Proteintech), PSMB6 (11684-2-AP, Proteintech), CYP2E1 (19937-1-AP, Proteintech), Tubulin (sc5286, Santa Cruz), AIF (17984-1-AP, Proteintech), EndoG (22148-1-AP, Proteintech), and cytochrome c (Cytc)

(6) Exosomal miR-184 in the aqueous humor of patients with central serous chorioretinopathy: a potential diagnostic and prognostic biomarkerJournal of NanobiotechnologyJuly 28, 2023Jee Myung Yang, Soo Jin Kim, Seongyeol Park, Wonyung Son, Anna Kim, Junyeop Leefor detection were rabbit anti-STC-2 (Abcam, #ab63057, US), and mouse anti-α-tubulin (Santa Cruz, #SC-5286, US), goat-anti-rabbit-HRP (Genetex, #GTX213110-01, US), and goat-anti-mouse-HRP(Genetex, #GTX213111-01,

(7) Identification of an Imidazopyridine-based Compound as an Oral Selective Estrogen Receptor Degrader for Breast Cancer TherapyCancer Research CommunicationsJuly 27, 2023Mengwu Pan, Valeria Solozobova, Nane C. Kuznik, Nicole Jung, Simone Gräßle, Victor Gourain, Yvonne M. Heneka, Christina A. Cramer von Clausbruch, Olaf Fuhr, Ravi S. N. Munuganti, et al.(Santa Cruz Biotechnology, catalog no. sc-365062, RRID:AB_10847862), α-Tubulin (Santa Cruz Biotechnology, catalog no. sc-5286, RRID:AB_628411), ERα (Santa Cruz Biotechnology, catalog no. sc-8002, RRID:AB_627558), GRP75/Mortalin

(8) Aging-related aneuploidy is associated with mitochondrial imbalance and failure of spindle assemblyCell Death DiscoveryJuly 8, 2023Fa-Li Zhang, Wei-Dong Li, Ke-Xin Zhu, Xu Zhou, Lan Li, Tin-Lap Lee, Wei Shenbovine serum albumin (BSA). The oocytes were then incubated in the anti-α-tubulin antibody (1:500; SC-5286; Santa Cruz Biotech, TX, USA), overnight at 4 °C. Subsequently, the oocytes were incubated for 1 h at

(9) The crescent-like Golgi ribbon is shaped by the Ajuba/PRMT5/Aurora-A complex-modified HURPCell communication and signaling : CCSJune 27, 2023Chiu SC, Yang XT, Wei TW, Liao YA, Chen JM, Kuo YC, Liu CJ, Cheng CY, Huang YJ, Huang YJ, et al.structure. Methods Antibodies, shRNA, plasmids, and reagents The following antibodies were used in the study: alpha tubulin (sc-5286), Golgin-160 (sc79966), actin (sc-8432), PRMT5 (sc-22132), Ajuba (sc-374610), GP73 (48010), and ARF1

(10) Polydeoxyribonucleotide exerts opposing effects on ERK activity in human skin keratinocytes and fibroblastsMolecular Medicine ReportsJune 20, 2023Sun Mee Shin, Eun Joo Baek, Kwang Ho Kim, Kwang Joong Kim, Eun Joo Parkphospho-p38 (sc-166182), p38α/β MAPK (sc-7972), phospho-JNK (sc-6254), JNK (sc-7345) and α-tubulin (sc-5286) purchased from Santa Cruz Biotechnology, Inc. The membranes were washed using Tris-buffered saline

(11) High-sensitivity calcium biosensor on the mitochondrial surface reveals that IP3R channels participate in the reticular Ca2+ leak towards mitochondriaPLOS ONEJune 9, 2023Yves Gouriou, Fabrice Gonnot, Mariam Wehbi, Camille Brun, Ludovic Gomez, Gabriel Bidauxprimary antibodies [rabbit anti-IP3R1 (Santa-Cruz, sc-28614; 1/500) and rabbit anti-TUBULIN (Santa-Cruz, sc-5286; 1/500)] (S1A Fig). Blots were incubated with horseradish peroxidase (HRP)-coupled sheep anti-mouse

(12) Indoleamine dioxygenase and tryptophan dioxygenase activities are regulated through control of cell heme allocation by nitric oxideThe Journal of biological chemistryJune 1, 2023Biswas P, Stuehr DJ.anti-iNOS (R & D systems # MAB9502; dilution 1:1000), and anti-α-Tubulin (Santa Cruz Biotechnology # sc-5286; dilution 1:2500). The proteins were detected using chemiluminescence using horseradish peroxidase–conjugated

(13) Elevated Nuclear PHGDH Synergistically Functions with cMyc to Reshape the Immune Microenvironment of Liver CancerAdvanced science (Weinheim, Baden-Wurttemberg, Germany)June 1, 2023Zhu H, Yu H, Zhou H, Zhu W, Wang X.monoclonal antibodies against cMyc (sc‐40, 1:2000 for IB, 1:200 for IHC, and 1:50 for ChIP), tubulin (sc‐5286, 1:2000 for IB), and CYP2B10 (sc‐73546, 1:50 for IHC) were purchased from Santa Cruz Biotech. A histone

(14) Nuclear Translocation of LDHA Promotes the Catabolism of BCAAs to Sustain GBM Cell Proliferation through the TxN Antioxidant PathwayInternational journal of molecular sciencesMay 27, 2023Li Z, Gu Z, Wang L, Guan Y, Lyu Y, Zhang J, Wang Y, Wang X, Xiong J, Liu Y.BCAT1 (Cell Signaling Technology, Cat.88785, 1:1000), Tubulin (Santa Cruz, Santa Cruz, CA, USA, Cat. sc-5286, 1:2000), Lamin B1 (Cell Signaling Technology, Cat.13435, 1:2000), β-actin (Santa Cruz, Cat. sc-047778,

(15) Overexpression of VIRMA confers vulnerability to breast cancers via the m(6)A-dependent regulation of unfolded protein responseCellular and molecular life sciences : CMLSMay 19, 2023Lee Q, Song R, Phan DAV, Pinello N, Tieng J, Su A, Halstead JM, Wong ACH, van Geldermalsen M, Lee BS, et al.Cell Signaling Technology cat#3179), ATF4 (1:1000; Cell Signaling Technology cat#11815), α-Tubulin (1:5000; Santa Cruz cat#sc-5286), LDHA (1:2500; Cell Signaling Technology cat#2012), LMNB1 (1;5000, Abcam cat#16048), GAPDH (1:5000;

(16) Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm CellsCancer Res CommunMay 1, 2023Saleiro D, Kosciuczuk EM, Fischietti M, Perez RE, Yang GS, Eckerdt F, Beauchamp EM, Hou Y, Wang Q, Weinberg RS, et al.RRID:AB_2136278) antibodies were from Cell Signaling Technology and anti-α-tubulin antibody (WB – 1:1,000, #sc-5286, RRID:AB_628411) was from Santa Cruz Biotechnology. Anti-GAPDH antibody (WB – 1:20,000, #MAB374, RRID:AB_2107445)

(17) zDHHC9 Regulates Cardiomyocyte Rab3a Activity and Atrial Natriuretic Peptide Secretion Through Palmitoylation of Rab3gap1JACC. Basic to translational scienceMay 1, 2023Essandoh K, Subramani A, Ferro OA, Teuber JP, Koripella S, Brody MJ.University of Iowa, 1:1,000),60,61 Rab27b (Synaptic Systems, 168103, 1:1,000), and α-tubulin (Santa Cruz, sc-5286, 1:500). Immunoblots were then incubated with the appropriate IRDye secondary antibody (LiCor Biosciences)

(18) Three-dimensional growth reveals fine-tuning of 5-lipoxygenase by proliferative pathways in cancerLife science allianceMay 1, 2023Göbel T, Goebel B, Hyprath M, Lamminger I, Weisser H, Angioni C, Mathes M, Thomas D, Kahnt AS.,Santa Cruz Biotechnology Raptor,sc-81537,Santa Cruz Biotechnology Rictor,sc-271081,Santa Cruz Biotechnology α-Tubulin,sc-5286,Santa Cruz Biotechnology α-Tubulin,ab176560,Abcam Antibodies used for immunoblot analysis.

(19) Epigenetic landscape reveals MECOM as an endothelial lineage regulatorNature CommunicationsApril 25, 2023Jie Lv, Shu Meng, Qilin Gu, Rongbin Zheng, Xinlei Gao, Jun-dae Kim, Min Chen, Bo Xia, Yihan Zuo, Sen Zhu, et al.dilution in PBST), GAPDH (Santa Cruz, #sc-32233, 1:1000 dilution in PBST), alpha Tubulin (Santa Cruz, #sc-5286, 1:1000 dilution in PBST), AP1(Signaling Technology, #9165, 1:1000 dilution in PBST), goat anti-rabbit

(20) PARP1 mediated PARylation contributes to myogenic progression and glucocorticoid transcriptional responseCell death discoveryApril 22, 2023Tan A, Younis AZ, Evans A, Creighton JV, Coveny C, Boocock DJ, Sale C, Lavery GG, Coutts AS, Doig CL.Abcam, UK), Myogenin (ab1835, Abcam, UK), NF-kB (8242, Cell Signalling Technology, UK), α-Tubulin (sc-5286, Santa Cruz Biotechnology, Inc., USA), and subsequently with HRP-conjugated anti-mouse or anti-rabbit

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(1)

2/23/2018alpha-Tubulin Antibody for Western Blot Andrea EstradaThis antibody was selected to function as a loading control to normalize protein expression to. The specific application I use it for is multiplexing with rabbit antibodies in order to normalize ... Read Review
(1)

12/30/2015Western Blot Analysis of Tubulin Lakshmi Reddy BolluThis antibody was used to detect tubulin in PC3 cells through western blot analysis. This antibody was purchased from Santa Cruz and detected band for tubulin in western blot.
(1)

12/28/2015Alpha Tubulin for Immunofluorescence Claire StewartThis was an experimental project to test whether we could visualize alpha tubulin under our fluorescent microscope.
(1)

10/6/2015Identifies alpha Tubulin in Western Blots Paul KimWe carry out subcellular fractionation and verify the isolated fractions by western blot analysis. We use a tubulin as a cytosolic marker.

American Journal of Respiratory Cell and Molecular Biology

Cell Death & Differentiation

α Tubulin (B-7) Antibody from Santa Cruz Biotechnology, Inc.

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Santa Cruz Biotechnology, Inc.

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α Tubulin (B-7) Antibody from Santa Cruz Biotechnology, Inc.

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