CD31/PECAM-1 Antibody AF3628 from R&D Systems, a Bio-Techne Brand

Product Specs
ItemCD31/PECAM-1 Antibody
CompanyR&D Systems, a Bio-Techne Brand
Price $529.00Supplier Page View Company Product Page
Catalog NumberAF3628
ApplicationsICC-IF,Ifs,IF/IHC,IHC fixed frozen,IHC-Pr,IHC-,Western Blot,Flow Cytometry,Immunohistochemistry,Western Blot,Immunohistochemistry-Paraffin,Immunohistochemistry-Frozen,In vivo assay,Simple Western,Immunofluorescence,Immunocytochemistry,CyTOF-ready,Functional Assay,Cell Culture,Cell Selection,Immunocytochemistry/ Immunofluorescence
Conjugate/TagUnconjugated
IsotypeIgG
HostGoat
Quantity100 ug (also 25 ug)
TargetCD31/PECAM-1
ReactivityGolden Syrian Hamster,Human,Mouse,Porcine,Primate,Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque),Rabbit,Rat,Rhesus Macaque,Transgenic Mouse,Xenograft
FormatLyophilized from a 0.2 µm filtered solution in PBS with Trehalose. See Certificate of Analysis for details. *Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS
ClonalityPolyclonal
ImmunogenMouse myeloma cell line NS0-derived recombinant mouse CD31/PECAM-1, Glu18-Lys590, Accession # Q08481
ConcentrationLYOPH
PurityAntigen Affinity-purified
Antibody TypePrimary Antibodies
StorageUse a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution

Specifications/Features

Available conjugates: Available conjugates: Unconjugated

Specificity: Detects human and mouse CD31/PECAM-1 in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant porcine CD31 is observed.

Sizes Available: 100 ug (also 25 ug)

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Questions and Answers

Can this CD31/PECAM-1 antibody be used for immunohistochemistry in mouse tissues?

Yes, the CD31/PECAM-1 antibody has been utilized in immunohistochemistry for mouse tissues, as evidenced by its application in studies examining vascular structures in various mouse models, including those related to brain development and chronic kidney disease (, ).

What is the recommended concentration for using this antibody in immunofluorescence assays?

The recommended concentration for the CD31/PECAM-1 antibody in immunofluorescence assays is typically around 10 μg/mL, as demonstrated in studies where it was used alongside other primary antibodies for co-staining (, ).

Does this antibody show cross-reactivity with other species?

The CD31/PECAM-1 antibody primarily detects human and mouse CD31/PECAM-1, with approximately 10% cross-reactivity observed with recombinant porcine CD31, making it suitable for studies involving these species ().

In what types of studies has this antibody been cited?

The CD31/PECAM-1 antibody has been cited in various studies focusing on vascular biology, including investigations into tumor microenvironments, chronic kidney disease, and developmental processes in the brain (, ).

R&D Systems, a Bio-Techne Brand

614 McKinley Place N.E.

Minneapolis, Minnesota 55413

United States

Phone: 1 (800) 343-7475
1 (612) 379-2956

Fax: 1 (612) 656-4400

Company Profile

Website: http://www.rndsystems.com

(1) Engineered human plasma-derived cryogels as a multifunctional scaffold to promote diabetic wound healingMaterials Today BioJune 1, 2026Yueming Zhao, Jiajun Hu, Kairui Duan, Tingting Li, Mian Lin, Bae Hoon Leemorphology and collagen deposition, respectively. Additionally, immunofluorescence staining for CD31 (AF3628, R&D Systems/biotechne) was carried out to assess vascularization. Primary antibodies against CD68 (83014-5-RR),

(2) Deep phenotyping of skin tissue remodeling in patients with systemic sclerosis treated with CD19-CAR T cellsNat CommunMay 23, 2026Aleix Rius Rigau, Meilin Xu, Ziyuan Liu, Sara Chenguiti Fakhouri, Janina Auth, Panagiotis Garantziotis, Andrea Zoli, Manoj Kumar Selvaraju, Maria Gabriella Raimondo, Carlo Tur, et al.Santa Cruz Biotechnology, Heidelberg, Germany). The primary antibodies used targeted CD31 (1:200, #AF3628; R&D Systems, Minneapolis, USA), Fibroblast activation protein α (FAP) (1:200, #AF3715; R&D Systems,

(3) Heterogeneity of estrogen and β-adrenergic receptors in female human coronary artery endothelial cellsInt J Cardiol Heart VascMay 15, 2026Basant Elsaid, Irakli Kopaliani, Ansam Seif, Stephan Speier, Andreas Deussenrabbit polyclonal antibody (1:200, Thermo Fisher Scientific, PA5-143874). CD31/PECAM-1 (R&D Systems, AF3628) was used as an endothelial marker across all wells. Two wells per slide were designated as controls,

(4) Pericytes are organ-specific regulators of tissue morphogenesisNat CommunMay 12, 2026Seyed Javad Rasouli, Kai Kruse, Rodrigo Diéguez-Hurtado, Parisa Ghanbari, Anusha Aravamudhan, Mara E. Pitulescu, Ralf H. AdamsMAB1179), rabbit anti-Aquaporin 5 (1:200, Millipore, 178615), goat anti CD31/PECAM1 (1:200, R&D Systems, AF3628), rat anti-PDGFRβ (1:100, eBioscience, 14-1402), goat anti-PDGFRβ (1:100, R&D Systems, AF1042), rabbit

(5) Beat-locked ATP microdomains in the sinoatrial node map a Ca2+-timed energetic hierarchy and regional pacemaker rolesJ Gen PhysiolMay 4, 2026Manuel F. Muñoz, Collin Matsumoto, Paula Rhana, Declan Manning, Geoanna M. Bautista, Daniel M. Collier, L. Fernando Santanaimmunolabeling, SA nodes were incubated for 48 h at 4°C with a goat anti-mouse CD31 primary antibody (1:50, AF3628; R&D Systems). After three 10-min PBS washes, tissues were incubated for 4 h at room temperature in

(6) TNFα and Endothelial IL-1 Receptor Signaling Drive Peritubular Capillary Regression and Fibrosis in Obstructive Kidney InjurybioRxivApril 27, 2026Charmain F. Johnson, Kate Wheeler, Jun Xie, Jenna Chan, George E. Davis, Courtney T. GriffinCell Signaling; #9661), mouse-anti-αSMA-Cy3 (1:250; Sigma; #C6198) and goat-anti-CD31 (1:100, R&D Systems; #AF3628). Secondary antibodies used were Cy5-donkey-anti-rabbit IgG (1:500, Jackson ImmunoResearch), and Cy3-donkey-anti-goat

(7) Inhibition of Astrocytic JMJD3 Attenuates Neuroinflammation-Mediated Blood-Brain Barrier Disruption and Improves Functional Recovery After Intracerebral Hemorrhage in MiceBrain SciApril 24, 2026Quan Zhang, Dewen Ru, Jiang Fang, Jun Zeng, Qiang Yuan, Zhuoying Du, Gang Wu, Jianhong Zhu, Jin Hupolyclonal antibody against platelet endothelial cell adhesion molecule 1 (CD31) (Catalogue Number: AF3628), rabbit polyclonal antibodies against claudin-5 (Catalogue Number: NBP2-92846) from Novus Biologicals

(8) CD47-mediated purification of human ventricular cardiomyocytes derived from pluripotent stem cellsAnim Cells Syst (Seoul)April 24, 2026Soon-Jung Park, Hyung Kyu Choi, Keon Ha Park, Na Kyeong Park, Sung-Hwan Moonstained with monoclonal antibodies against cTnT (Invitrogen, MA5-12960) (1:500), CD31 (R&D Systems, AF3628) (1:200), and sarcomeric-α-actinin (Abcam, ab137346) (1:200) diluted in 0.03% Triton X-100 and incubated

(9) Glioma-intrinsic SLC1A3 hijacks the vascular niche to establish an immunosuppressive microenvironmentFrontiers in ImmunologyApril 23, 2026Hao Lin, Chaxian Liu, Xi Chen, Yingbo Zhao, Yi Lyu, Bilong Zhang, Haikun Song, Xiaomin Fan, Shasha Li, Ziqian He, et al.blocked for 2h at RT, and primary antibodies were incubated at 4 °C overnight (CD31 1:300, R&D Systems, AF3628; HA 1:300, CST, #2367; SLC1A3, 1:300, Proteintech, 20785-1-AP). The secondary antibodies were incubated

(10) Spatial Protein Expression Analysis in Lungs Using Capillary-Based Immunoassay After Laser-Assisted MicrodissectionCellsApril 21, 2026Stefan Hadzic, Marija Gredic, Vanessa Nebel, Norbert Weissmann, Cheng-Yu Wuwere then incubated with goat anti-platelet and endothelial cell adhesion molecule 1 (PECAM1; 1:200, AF3628, R&D system, Bio-Techne, Minneapolis, MN, USA), mouse anti-alpha-smooth muscle actin (α-SMA; 1:200,

(11) Brain-Derived Cystathionine β-Synthase-Generated H2S Attenuates Cerebral Ischemia-Reperfusion Injury via VEGFR2-Mediated Angiogenesis in MCAO/R RatsCurr Issues Mol BiolApril 18, 2026Shuai Liang, La Jiang, Yu Jiang, Shan Wang, Jia-Rong Jiang, Ji-Yue Wen, Zhi-Wu Chen, Shuo ChenYuanye Biotech, Shanghai, China); DAPI mounting medium (P0131, Beyotime, Shanghai, China); anti-CD31 (AF3628) and anti-VEGFR2 (AF644, R&D Systems, Minneapolis, MN, USA); anti-CBS (DF6410), ZO-1 (AF5145) and Claudin 5

(12) Galectin-3 mediates lysosome-related inflammation within monocyte-derived macrophages in a mouse model of ischemic brain injuryJournal of Clinical InvestigationApril 15, 2026Miao Wang, Zhentai Huang, Zhihong Du, Jiajing Shan, Qing Ye, Lingxiao Lu, Ming Jiang, Fei Xu, Ziyang Liu, David J.R. Fulton, et al.anti-GFAP (Invitrogen, 13-0300), rat anti-CD68 (Invitrogen, 14-0681-82), goat anti-CD31 (R&D Systems, AF3628), goat anti-MPO (R&D Systems, AF3667), mouse anti-GAL3 (Santa Cruz Biotechnology, sc-32790), rabbit

(13) Tet2-driven clonal hematopoiesis drives aortic aneurysm via macrophage-to-osteoclast-like differentiationJournal of Clinical InvestigationApril 15, 2026Jun Yonekawa, Yoshimitsu Yura, Junmiao Luo, Katsuhiro Kato, Shuta Ikeda, Yohei Kawai, Tomoki Hattori, Ryotaro Okamoto, Mari Kizuki, Emiri Miura-Yura, et al.anti–rabbit CD68 (E307V) (catalog 97778, Cell Signaling Technology), and anti–goat CD31/PECAM-1 (catalog AF3628, R&D Systems). The secondary antibodies included donkey anti–rabbit Alexa Fluor 488 (catalog A21206,

(14) Gelatin Sponge-Embedded Adipose-Derived Stromal Cells Enable Allogeneic Application for Revascularization of Ischemic WoundsInt J Mol SciApril 13, 2026Manon Locatelli, Wolf-Henning Boehncke, Damien Pastor, Jean Villard, Nicolo-Constantino Brembilla, Olivier Preynat-Seauve(Abcam ab16669, 1/200, Cambridge, MA, USA) or goat anti-rat CD31 (R&D Systems, Minneapolis, MN, USA, AF3628, 1/200). For immunofluorescence (CD3), sections were stained with donkey anti-rabbit IgG-Alexa 555 antibody

(15) ADAMTS-7 Increase Contributes to Worsened Neurological Outcome in Mice on High Fat DietCNS Neurosci TherApril 12, 2026Yang Xu, Jun Li, Zhiyi Zuonumber: 66003–1‐Ig, Proteintech) and cluster of differentiation 31 (CD31) (1:200, catalogue number: AF3628, R&D Systems). After being washed, sections were incubated for 1 h at room temperature with secondary

(16) Impaired vitamin D signaling reveals neutrophils as key drivers of prostate cancer disseminationEMBO Molecular MedicineApril 10, 2026Kateryna Len-Tayon, Justine Gantzer, Charles Dariane, Olivier Fogel, Vanessa Friedrich, Daniela Rovito, Véronique Lindner, Valentine Gilbart, Darya Yanushko, Sandrine Henri, et al.anti-CD45 (Abcam Cat# ab10558, RRID:AB_442810; 1:600 dilution), goat anti-CD31 (R and D Systems Cat# AF3628, RRID:AB_2161028; 1:200 dilution), rabbit anti-F4/80 (Cell Signaling Technology Cat# 70076, RRID:AB_2799771;

(17) Injection site dictates the immune response to a biodegradable polymer and corresponding collagen regenerationBioact MaterApril 8, 2026Yanshuang Zhang, Tao Sun, Wenqian Li, Ye Fu, Yue Yu, Runjia Shen, Jiaqi Li, Lin Yu, Jiandong Dingovernight at 4 °C with primary antibodies targeting vimentin, CD68 (ABclonal, A20803), CD31 (R&D SYSTEMS, AF3628), HMGB1 (Cell Signaling Technology, 3935), HSP70 (ABclonal, A23457), NF-κB p65 (ABclonal, A19653), CD206

(18) Olink Proteomics Reveals CCL2 Aggravates Perihematomal Edema After Intracerebral Hemorrhage in High-Altitude Migrants Via CCR2/NF-κB-Mediated Blood-Brain Barrier DisruptionCNS Neurosci TherApril 7, 2026Rongsu Huang, Ling Gao, Yalan Dai, Shangshi Li, Jiancai Guru, Mingxi Li, Yù-Jié Chen, Bo Wu, Xufang Ru, Qing Gong, et al.(2H5; 14–7096‐81; Invitrogen), anti‐Iba1 (GTX100042; GeneTex), anti‐ZO‐1 (PB9234; Boster), anti‐CD31 (AF3628; Bio‐Techne), anti‐GFAP (P14136; Bioworld), and anti‐CCR2 (PA5‐23037; Invitrogen). After being washed,

(19) Anti-PECAM-1 antibodies: another tool for visualization of Reissner´s fiber and the subcommissural organ in rat central nervous systemBrain Struct FunctApril 4, 2026Lukáš Malčický, Ján Košuth, Daniel Barčák, Jarmila Zrubáková, Marie Vancová, Zuzana Daxnerová, Juraj Ševc, Anna Alexovič Matiašovápolyclonal,"Mouse CD31/PECAM-1 aa 18–590 (Q08481), recombinant (extracellular region)",R&D systems/bio-techne,AF3628,AB_2161028,1:500 (IF) 1:20 (IEM) Anti-CD31 [EPR17260-263],Rabbit monoclonal,"Mouse CD31 aa 400–600

(20) SELL Marks an Effector-Deficient CD8+ T Cell Subset That Promotes Intracerebral Hemorrhage and Responds to Rutin TherapyHum MutatApril 2, 2026Yan Huang, Haochen Xu, Congxia Bai, Jing Liu, Yingying Sun, Jingzhou Chenantibody (Sigma‐Aldrich C6198, clone 1A4), goat anti‐Iba‐1 (Abcam ab5076), and goat anti‐CD31 (R&D Systems AF3628). Secondary detection employed Alexa Fluor–conjugated donkey immunoglobulins: antigoat IgG‐594, antirat

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(1)

5/2/2022Great CD31 Antibody for IF Efstathios StamatiadesWe are interested to study tissue-resident macrophages and their interplay with endothelial cells.
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2/8/2022CD31 Antibody for Immunofluorescence Bhavin ShahExcellent antibody for marking the vasculature in tissue, sections, and slices.
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9/18/2020Goat anti-CD31/PECAM1 José VieiraHigh quality antibody to label endothelial cells. Low background is obtained.
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11/30/2016Use Of CD31 (PECAM-1) Antibody From R&D Systems On Differentiated Embryonic Stem Cell Culture J ChalThis antibody was used to analyze the presence of PECAM-1 positive endothelial cells in differentiated culture of mouse embryonic stem cells (mESCs). This polyclonal (goat) anti-CD31 antibody appears ... Read Review