BioLegend
Purified anti-DYKDDDDK Tag Antibody
637301
L5
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I specialize in functional genomics and applying gene editing technologies to address immunology questions. I have recently developed a new barcoding system, which represents the largest known to date set of protein-based reporters (doi.org/10.1016/j.cell.2018.09.022). I used this antibody to detect FLAG-tagged recombinant protein expressed by various cell lines (HEK 293T, 4T1, Jurkat, THP1 etc.) by flow (APC, cat # 637307) and mass cytometry (directly conjugated with a metal isotope). The antibody works excellent with some background signal.
Mass cytometry and flow cytometry
Human HEK-293T cells transduced with FLAG-tagged recombinant protein
1:600, 30 min on ice; for flow antibody (APC): 1:300
Fc blocking reagent (BioLegend)
N/A
CyTOF 2; BD Fortessa
Excellent antibody to detect FLAG-tagged recombinant proteins.
doi.org/10.1016/j.cell.2018.09.022
Great staining
Highly recommended! Better than other FLAG antibodies I tried before (such as clone 5A8E5 from GenScript).