Good Purified Anti-DYKDDDDK Tag Antibody from BioLegend

Genetics and Genomic Sciences
Mount Sinai
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified anti-DYKDDDDK Tag Antibody

Catalog Number:

637301

Clone Number:

L5

I specialize in functional genomics and applying gene editing technologies to address immunology questions. I have recently developed a new barcoding system, which represents the largest known to date set of protein-based reporters (doi.org/10.1016/j.cell.2018.09.022). I used this antibody to detect FLAG-tagged recombinant protein expressed by various cell lines (HEK 293T, 4T1, Jurkat, THP1 etc.) by flow (APC, cat # 637307) and mass cytometry (directly conjugated with a metal isotope). The antibody works excellent with some background signal.

Experimental Design and Results Summary

Applications

Mass cytometry and flow cytometry

Sample

Human HEK-293T cells transduced with FLAG-tagged recombinant protein

Primary Incubation

1:600, 30 min on ice; for flow antibody (APC): 1:300

Blocking Agent

Fc blocking reagent (BioLegend)

Secondary Incubation

N/A

Tertiary Incubation

N/A

Detection

CyTOF 2; BD Fortessa

Results Summary

Excellent antibody to detect FLAG-tagged recombinant proteins.

DOI or PMID #

doi.org/10.1016/j.cell.2018.09.022

Additional Notes

N/A

Related Categories

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Summary

The Good

Great staining

The Bad

N/A

The Bottom Line

Highly recommended! Better than other FLAG antibodies I tried before (such as clone 5A8E5 from GenScript).

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