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APC Annexin V
APC Annexin V from BioLegend
(2)
Write a review
Citations:
(132)
Supplier Page
Supplier Page from
BioLegend for
APC Annexin V
Description
APC Annexin V
Product Specs
Company Info
Citations
Reviews
Product Specs
Item
APC Annexin V
Company
BioLegend
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
640920
Quantity
100 tests
Applications
Flow Cytometry (FC)
Conjugate/Tag
APC
NCBI Full Gene Name
annexin A5
NCBI Gene Aliases
ANX5, CPB-I, ENX2, HEL-S-7, PP4, RPRGL3, VAC-alph
Format
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Target
Annexin V
Species
Human, Mouse, Rat
Add to Compare List
BioLegend
8999 BioLegend Way
San Diego
,
California
92121
United States
Phone
: 1-877-246-5343 Toll-Free, USA & Canada
1-858-768-5800 International
Fax: 1-877-455-9587
Company Profile
Website:
biolegend.com
Citations (132)
(1)
End-binding protein 1 regulates the metabolic fate of CD4+ T lymphoblasts and Jurkat T cells and the organization of the mitochondrial network
Frontiers in Immunology
July 13, 2023
Álvaro Gómez-Morón, Silvia Requena, Clara Pertusa, Marta Lozano-Prieto, Diego Calzada-Fraile, Camila Scagnetti, Inés Sánchez-García, Ana Adela Calero-García, Manuel Izquierdo, Noa B. Martín-Cófreces
(
Tonbo biosciences
) in 100 µl PBS during 30 min at 4°C and with 2 µl APC-conjugated annexin V (No.
640920
Biolegend
) in 100 µl
Annexin V Binding Buffer
(No.
422201
,
Biolegend
) for 15 min at RT, following the
(2)
Role of mitochondria in the myopathy of juvenile dermatomyositis and implications for skeletal muscle calcinosis
Journal of Autoimmunity
July 1, 2023
Bhargavi Duvvuri, Lauren M. Pachman, Payton Hermanson, Ting Wang, Richard Moore, Dennis Ding-Hwa Ding-Hwa Wang, Aaron Long, Gabrielle A. Morgan, Stephen Doty, Rong Tian,
et al.
analysis (
flow cytometry
) Viability analysis was done using APC-conjugated Annexin V (
Biolegend
Cat. No.
640920
and propidium iodide (
Miltenyi Biotec
Cat. No.
130–093-233
). Briefly, adhered RH30 cells following trypsinization
(3)
Stepwise activities of mSWI/SNF family chromatin remodeling complexes direct T cell activation and exhaustion
Molecular cell
April 20, 2023
Battistello E, Hixon KA, Comstock DE, Collings CK, Chen X, Rodriguez Hernaez J, Lee S, Cervantes KS, Hinkley MM, Ntatsoulis K,
et al
.
IFN-γ (
BioLegend
, Cat#
505825
). Annexin
staining
was performed with
APC Annexin V
(
BioLegend
, Cat#
640920
). Samples were analyzed using a Fortessa cytometer.
Chromatin
-focused
CRISPR
screen Library design For
(4)
Small-Molecule Cyclophilin Inhibitors Potently Reduce Platelet Procoagulant Activity
International Journal of Molecular Sciences
April 12, 2023
Jens Van Bael, Aline Vandenbulcke, Abdelhakim Ahmed-Belkacem, Jean-François Guichou, Jean-Michel Pawlotsky, Jelle Samyn, Arjan D. Barendrecht, Coen Maas, Simon F. De Meyer, Karen Vanhoorelbeke,
et al.
(HEPES)-buffered saline (pH 7.3) in the presence or absence of 2.5 mM CaCl2 and AnnexinV (APC; cat. n°
640920
,
Biolegend
, San Diego, CA, USA). Anti-
CD41
(BV421; Biolegend, San Diego, CA, USA) or anti-
CD42b
(Super
(5)
Expansion of circulating stem-like CD8+ T cells by adding CD122-directed IL-2 complexes to radiation and anti-PD1 therapies in mice
Nature Communications
April 12, 2023
Kateryna Onyshchenko, Ren Luo, Elena Guffart, Simone Gaedicke, Anca-Ligia Grosu, Elke Firat, Gabriele Niedermann
(
eBiosciences
,
88-8824-00
) according to the manufacturer’s instructions. The APC Annexin V/PI Kit (
BioLegend
,
640920
) was used to determine the proportion of apoptotic cells. Data were acquired using a CytoFlex S Flow
(6)
Niche stiffness sustains cancer stemness via TAZ and NANOG phase separation
Nature communications
January 16, 2023
Liu X, Ye Y, Zhu L, Xiao X, Zhou B, Gu Y, Si H, Liang H, Liu M, Li J,
et al
.
incubated with 100 μL binding buffer (Biolegend) containing 5 μL APC-conjugated Annexin V (
1:20
, cat#
640920
,
Biolegend
) for 15 min at room temperature. After
incubation
, the cells were washed and resuspended
(7)
Phosphoproteomic profiling of T cell acute lymphoblastic leukemia reveals targetable kinases and combination treatment strategies
Nature Communications
December 1, 2022
Valentina Cordo’ et al.
staining
of apoptotic cells, 200,000 cells were stained with Annexin V-APC antibody (
Biolegend
cat#
640920
) diluted
1:20
in
Annexin V-binding buffer
(
Invitrogen
cat#
V13246
) for 15 min at room temperature (RT)
(8)
Inhibition of SRPK1, a key splicing regulator, exhibits antitumor and chemotherapeutic-sensitizing effects on extranodal NK/T-cell lymphoma cells
BMC cancer
October 27, 2022
He C, Liu B, Wang HY, Wu L, Zhao G, Huang C, Liu Y, Shan B, Liu L.
resistance.
Flow cytometry
assay
Annexin V (
420404
,
Biolegend
, USA) and 7-amino-actinomycin D (7-AAD,
640920
,
Biolegend
, USA) double
staining
was performed to detect cell apoptosis. Cells were analyzed using the
(9)
G6PD promotes cell proliferation and dexamethasone resistance in multiple myeloma via increasing anti-oxidant production and activating Wnt/β-catenin pathway
Experimental hematology & oncology
October 21, 2022
Li R, Ke M, Qi M, Han Z, Cao Y, Deng Z, Qian J, Yang Y, Gu C.
Subsequently, cell pellets were resuspended in 100 μL 1 × Binding buffer, to which 5 μL Annexin V-APC (#
640920
,
Biolegend
, Beijing, China) and 5 μL PI (50 μg/mL, #25535-16-4, Solarbio Life Science, Beijing, China)
(10)
Polyethylenimine, an Autophagy-Inducing Platinum-Carbene-Based Drug Carrier with Potent Toxicity towards Glioblastoma Cancer Stem Cells
Cancers (Basel)
October 15, 2022
Conor McCartin, Candice Dussouillez, Chloé Bernhard, Eric Mathieu, Juliette Blumberger, Monique Dontenwill, Christel Herold-Mende, Ahmed Idbaih, Philippe Lavalle, Stéphane Bellemin-Laponnaz,
et al.
140 mM NaCl, 2.5 mM CaCl2, pH 7.3–7.4). Triplicates were pooled and stained with APC-conjugated AnV (#
640920
,
BioLegend
, Amsterdam, Netherlands) diluted 1/100 with prepared AnV binding buffer which was incubated
(11)
Tumorous expression of NAC1 restrains antitumor immunity through the LDHA-mediated immune evasion
Journal for ImmunoTherapy of Cancer
September 23, 2022
Yijie Ren et al.
(St Louis, MI). The apoptosis analysis was performed using
APC Annexin V
Apoptosis Detection
Kit (#
640920
) with the Aqua Live/Dead (#
423101
) from
BioLegend
(San Diego, California, USA). Glycolysis analysis For
(12)
A Planar Culture Model of Human Absorptive Enterocytes Reveals Metformin Increases Fatty Acid Oxidation and Export
Cellular and Molecular Gastroenterology and Hepatology
April 28, 2022
Ismael Gomez-Martinez, R. Jarrett Bliton, Keith A. Breau, Michael J. Czerwinski, Ian A. Williamson, Jia Wen, John F. Rawls, Scott T. Magness
anti-
human
hashtag 6 antibody,"BioLegend (San Diego, CA)",
394641
Annexin V-APC,"
BioLegend
(San Diego, CA)",
640920
RNAqueous-Micro Total RNA Isolation Kit
,"
Thermo Fisher
(Waltham, MA)",
AM1931
"Chromium Next GE M Single-Cell
(13)
Oncolytic Herpes Simplex Virus Type 1 Induces Immunogenic Cell Death Resulting in Maturation of BDCA-1(+) Myeloid Dendritic Cells
International journal of molecular sciences
April 27, 2022
Kalus P, De Munck J, Vanbellingen S, Carreer L, Laeremans T, Broos K, Dufait I, Schwarze JK, Van Riet I, Neyns B,
et al
.
were resuspended in 100 µL composed of 2 µL AnnexinV-APC (1/50;
BioLegend
, Amsterdam, Netherlands;
640920
), 3.3 µL
DAPI solution
(0.03 µg/mL) and 94.7 µL 1X Annexin V Binding Buffer (
BD Biosciences
, 55645).
(14)
Ca
2+
-activated sphingomyelin scrambling and turnover mediate ESCRT-independent lysosomal repair
Nature communications
April 6, 2022
Niekamp P, Scharte F, Sokoya T, Vittadello L, Kim Y, Deng Y, Südhoff E, Hilderink A, Imlau M, Clarke CJ,
et al
.
µM ionomycin or 0.1% (v/v) DMSO for 10 min at 37 °C in 5% CO2. Next,
APC-Annexin V
(
Biolegend
, no.
640920
; 5 µl in 100 µl Binding Buffer) and propidium iodide (5 µg/ml;
Sigma-Aldrich
,
P4170
) were added and
(15)
Hematopoietic stem cells temporally transition to thrombopoietin dependence in the fetal liver
Science advances
March 18, 2022
Lee Y, DiMaulo-Milk E, Leslie J, Ding L.
(1 μg/ml). Annexin V
staining
was performed using Annexin V Apoptosis Detection Kit APC (
BioLegend
,
640920
) per the manufacturer’s instructions. Intracellular
staining
For intracellular
staining
, cells were
(16)
Integrated Multiomic Profiling Identifies the Epigenetic Regulator PRC2 as a Therapeutic Target to Counteract Leukemia Immune Escape and Relapse
Cancer discovery
March 7, 2022
Gambacorta V, Beretta S, Ciccimarra M, Zito L, Giannetti K, Andrisani A, Gnani D, Zanotti L, Oliveira G, Carrabba MG,
et al
.
V–positive cells at the end of the
assay
within the population of blasts (
BioLegend
,
APC Annexin V
; cat. #
640920
, lot #B334668). Untreated target cells served as a control to detect the basal proportion of apoptotic
(17)
Kupffer Cells and Blood Monocytes Orchestrate the Clearance of Iron-Carbohydrate Nanoparticles from Serum
International journal of molecular sciences
February 28, 2022
Arsiwala T, Vogt AS, Barton AE, Manolova V, Funk F, Flühmann B, Bachmann MF.
Franklin Lakes, NJ, USA), anti-
Ly6G
(#
17-9668-80
,
Thermo Fisher Scientific
, Waltham, MA, USA), annexin V (#
640920
,
BioLegend
San Diego, CA, USA), and Fixable viability dye (eFluorTM506 CYAN, #
65-0866-14
,
eBioscience
,
(18)
Specific overexpression of SIRT1 in mesenchymal stem cells rescues hematopoiesis niche in BMI1 knockout mice through promoting CXCL12 expression
International Journal of Biological Sciences
February 28, 2022
Jinbo Li, Xing Li, Wen Sun, Jiao Zhang, Quanquan Yan, Jun Wu, Jianliang Jin, Ruinan Lu, Dengshun Miao
analysis. To detect Annexin V,
CD44
and
CXCR4
expression on LSK surfaces, APC-conjugated Annexin V (#
640920
) or
CD44
(#
103012
) or
CXCR4
(#
146508
) from
BioLegend
were added in
staining
panel with LSK surface markers
(19)
A Proximal-to-Distal Survey of Healthy Adult Human Small Intestine and Colon Epithelium by Single-Cell Transcriptomics
Cellular and molecular gastroenterology and hepatology
January 1, 2022
Burclaff J, Bliton RJ, Breau KA, Ok MT, Gomez-Martinez I, Ranek JS, Bhatt AP, Purvis JE, Woosley JT, Magness ST.
RNAscope Multiplex Fluorescent Reagent Kit
v2,"
Advanced Cell
Diagnotics (Newark, CA)",323100 Antibodies,, AnnexinV-APC,
BioLegend
,
640920
Lysozyme,"Diagnostic Biosystems (Pleasanton, CA)",RP028 CFTR,
Cystic Fibrosis
Foundation,A570 Mucin
(20)
Red blood cells and their releasates compromise bone marrow-derived human mesenchymal stem/stromal cell survival in vitro
Stem Cell Research & Therapy
December 1, 2021
Ryan Christopher Dregalla, Jessica Ann Herrera, Edward Jeffery Donner
FITC (
Biolegend
, cat. #
328108
) to gate for MSCs, annexin-V-conjugated (AV) to APC (
Biolegend
, cat. #
640920
) to detect apoptotic cells and 7-aminoactinomycin D (7AAD) viability stain (
Invitrogen
, cat. #
00-6993-50
)
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Reviews
Write a Review
(1)
8/6/2018
Annexin V APC for Determining Apoptosis in Cells
Suzanne Tomchuck
We often look at the effects of different compounds on cell viability and apoptosis. We typically use Annexin V in combination with 7AAD or PI, to determine apoptosis by flow cytometry. We prefer the...
Read Review
(1)
2/24/2017
Staining T Cells With Annexin V APC
Adam Guess
This reagent was used to characterize living versus apoptotic/necrotic cells. It was chosen because it is the gold standard for measuring apoptosis, APC is bright and works well with other ...
Read Review
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APC Annexin V from BioLegend
Supplier Page
View Company Product Page
Pricing Info
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