Anti-Mouse Gr1 BV421 for Splenocyte Staining

January 04, 2016

Tisch Cancer Institute
Icahn School of Medicine at Mount Sinai
Post-Doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Brilliant Violet 421™ anti-mouse Ly-6G/Ly-6C (Gr-1) Antibody

Catalog Number:

108434

This product was used to identify Gr1+ cells amongst splenocytes (eg neutrophils or monocytes) under different conditions (eg tumor bearing vs non tumor bearing mice)

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes

Primary Incubation

1:400, 15min, 4C

Blocking Agent

Blocking Buffer containing sera (rat, rabbit, human, goat)

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

Flow cytometry (BD) LSRFortessa

Results Summary

Excellent signal to noise ratio for this antibody

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright fluorophore

The Bad

No complaints

The Bottom Line

Easy identification of Gr1+ cells in spleen

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