Purified Anti-DYKDDDDK - Great Product

March 06, 2019

Markey Cancer Center
University of Kentucky
Principal Investigator

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

Purified anti-DYKDDDDK

Catalog Number:

637304

This is the best FLAG antibody I have ever used. Great for WB and IP.

Experimental Design and Results Summary

Applications

Immunoprecipitation

Sample

FLAG tagged proteins expressed in CHO-K1 cells

Primary Incubation

1–2 hr

Blocking Agent

5% milk in TBST

Secondary Incubation

1 hr

Tertiary Incubation

N/A

Detection

LiCO Odyssey

Results Summary

Clear single band was detected

DOI or PMID #

N/A

Additional Notes

I used goat anti-Rat secondary from LiCo

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Summary

The Good

Specific and more sensitive than other brands

The Bad

Need to have goat anti-rat IgG

The Bottom Line

Great antibody for WB and IP.

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