Fast, Easy and Multipurpose Fluorometer

UNC Chapel Hill

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

Qubit 4

Catalog Number:

Q33238

We use this regularly in the lab to measure protein, RNA and DNA (almost daily). It is a small, compact machine that takes up very less space. It can also measure protein quickly which saves me a lot of time and I don't have to do long assays!

Experimental Design and Results Summary

Application

RNA, DNA and protein quantification

Starting Material

N/A

Tips

Follow the incubation time as stated on the protocol for each type of sample and mix well.

Results Summary

I use this for RNA, DNA and protein quantification, which I usually can detect at very low concentrations. Also, the volume needed for detection is as low as 1ul. So the sample does not get wasted!

DOI or PMID #

N/A

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Summary

The Good

Faster than other assays (specially for protein), more accurate than UV absorbance, very less sample is needed, I can trust my quality of sample after measuring it from this equipment (they use two different dyes for RNA and use the ratio of it to calculate the amount of RNA degraded).

The Bad

It is expensive, even the dyes that are used with it are pricey.

The Bottom Line

Everybody in the lab uses this regularly and we really like it.

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