Efficient ß-Tubulin Antibody for Western Blot

June 28, 2022

Biology of Aging
MC
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

β-Tubulin antibody

Catalog Number:

2146

Clone Number:

N/A

This antibody was used as a loading control for western blot analysis. Cell lysates (25 ug/mL protein) were used.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cells

Primary Incubation

1:1000, overnight@4ºC

Blocking Agent

5% BSA/TBS

Secondary Incubation

Anti-rabbit IgG HRP (#HAF008, R&D systems), 1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescent

Results Summary

The antibody provides robust results with bands appearing at 55 kDa with minimal background. It can be reused more than 5 times when kept at 4ºC in BSA/TBS solution.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Sensitive, robust, dilution in BSA/TBS can be reused.

The Bad

None

The Bottom Line

Great antibody for western blot analysis in human cell lysates.

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