Sensitive P16 INK4A Rabbit mAb for Western Blot

June 27, 2022

Biology of Aging
MC
Research Scientist

Overall

Quality of Results

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Company:

Cell Signaling Technologies

Product Name:

P16 INK4A rabbit mAb

Catalog Number:

92803

Clone Number:

D3W8G

p16 is a tumor suppressor protein that plays an important role in regulating the cell cycle. This antibody was used to detect p16 levels in human cell lysates through western blot analysis.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human cells

Primary Incubation

1:500, overnight@4ºC

Blocking Agent

5% BSA/TBS

Secondary Incubation

Anti-rabbit IgG HRP (#HAF008, R&D systems), 1:5000

Tertiary Incubation

N/A

Detection

Chemiluminescent

Results Summary

Western Blot analysis was conducted with 25 ug/mL of protein. The antibody is really sensitive and provides robust results with low exposures.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Sensitive, consistent

The Bad

None

The Bottom Line

Really good antibody; detects low levels of p16 in human cell samples.

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