Effect of Chemoattractant on Thioredoxin-1 Expression

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Company:

Cell Signaling

Product Name:

Thioredoxin 1 (C63C6) Rabbit mAb

Catalog Number:

2429

Clone Number:

N/A

Trx1is a 12-kD multifunctional antioxidant protein with a redox-active disulfide/dithiol within the conserved active site Cys32–Gly–Pro–Cys35. The Trx system composed of Trx and TrxR1 (Trx reductase)is an effective protein disulfide reductase that regulates the redox status of the cell. Here we studied the time course effect of Thioredoxin-1 expression by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse Lysate

Primary Incubation

1:1000 prepared in 1x TBST

Blocking Agent

5% Skim milk

Secondary Incubation

1:4000 prepared in 1x TBST

Tertiary Incubation

None

Detection

ECL

Results Summary

We performed western blot and detect, analyzed the Trx-1 expression. We found that there is a a time dependent increase of Trx-1 expression by chemoattractant.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

This antibody works great for western blot.

The Bad

None

The Bottom Line

This antibody works great for western blot, immunoprecipitation and also for microscopy. Highly recommended.

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