Tocris Bioscience
2-APB
1224
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HEK293 cells were incubated with intracellular Ca2+ sensitive dye, Fura-2AM in normal tyrode solution for 40 min, washed and kept aside for 20 min. Fura2-loaded cells were excited alternately at 340 and 380 nm and fluorescence at 510 nm was expressed as 340/380 ratio. Intracellular Ca2+ stores were depleted with 30 µM BHQ which promotes store-operated Ca2+ entry into the cells upon the addition of 1 mM extracellular Ca2+. Preincubation of cells with 50 µM 2-APB for 10 min reduces this Ca2+ entry.
Inhibition of store-operated Ca2+ entry in HEK293 cells.
HEK293 cells, Fura2-AM dye, BHQ
Dissolve 2-APB in DMSO and store in aliquots at -20 degree celcius.
Preincubation of cells with 50 μM 2-APB for 10 min inhibited store-operated Ca2+ entry following store depletion with BHQ in HEK293 cells.
N/A
Efficient in inhibiting store-operated Ca2+ entry.
Not specific at highter concentrations.
2-APB inhibits store-operated Ca2+ entry in HEK293 cells.