Excellent Antibody to Detect Caspase-3

Pharmacology
PennState College of Medicine
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore Sigma

Product Name:

Anti-Caspase 3 Antibody, active (cleaved) form

Catalog Number:

AB3623

Clone Number:

N/A

Info:Supplier Page 

Browse Similar Products: Caspase 3 Antibodies

This antibody works really well and it's a good antibody to assess cell death by detecting Caspase-3 levels. Very good antibody to assess apoptosis via western blotting.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mammalian cell line whole cell lysate

Primary Incubation

1:200, overnight incubation at 4 degree Celsius

Blocking Agent

5% BSA in TBST

Secondary Incubation

Goat anti-rabbit IgG HRP Ab, dilution (1:2500) in 5% BSA , 1hr at RT

Tertiary Incubation

N/A

Detection

N/A

Results Summary

Antibody reacted very well, without many non-specific bands. We were able to determine caspase-3 activity by western blotting.

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Quality of the results.

The Bad

None

The Bottom Line

Very good antibody to assess apoptosis via western blotting.

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