Excellent Antibody for Flow Cytometry of Tissue Resident Memory Cells in Human Tissues

June 28, 2022

Infectious Diseases
Vall d'Hebron Institute of Research
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

anti-CD49a-PE-Cy7 Ab

Catalog Number:

328312

Clone Number:

TS2/7

We use this antibody to study the phenotype of TRM NK cells in several tissues. It stains better than desired.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human tonsils

Primary Incubation

0.6 ul in 100ul of Staining Buffer (3% FBS in PBS 1x). 20min at RT

Blocking Agent

Staining buffer

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR Fortessa

Results Summary

It worked to study the phenotype of tissue resident memory NK cells in human tonsils. The gate of CD49a is easy to set when combined with other markers such as CD69 or CD103.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Excellent staining.

The Bad

None

The Bottom Line

Antibody with great signal, so you can titrate it.

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