Excellent Anti-Human CD45-FITC From BioLegend for Flow

November 28, 2023

College of Medicine
University of Tennessee HSC
Postdoctoral Researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

BioLegend

Product Name:

FITC anti-human CD45

Catalog Number:

982316

Clone Number:

HI30

For immunophenotyping of different types of cells present in human blood, I used an anti-human CD45-FITC antibody in my master mix to detect all nucleated hematopoietic cells.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human blood

Primary Incubation

1:50

Blocking Agent

Fc-block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometer

Results Summary

A clear cloud of cells was detected in FITC channel indicating cells expressing surface marker CD45.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Good signals with great separation between positive and negative population.

The Bad

None.

The Bottom Line

Small quantity of antibody has high stain index, prefer to use mostly in my master mixes.

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