Good Anti-Human HLA-DR-BV421 Antibody From BioLegend Ideal for Flow

College of Medicine
University of Tennessee HSC
Postdoctoral Researcher

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Company:

BioLegend

Product Name:

Brilliant Violet 421 anti-human HLA-DR antibody

Catalog Number:

307635

Clone Number:

L243

To check the increase in the frequency of dendritic cells in human PBMCs collected from respiratory virus-infected patients, I have used this antibody in the immunophenotype antibody cocktail.

Experimental Design and Results Summary

Applications

Flow cytometry

Sample

Human PBMCs

Primary Incubation

2.5 ul in 100 ul test reaction

Blocking Agent

Fc-block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometer

Results Summary

Good percent and separate cloud of cells were recorded in BV421 channel indicating HLA-DR positive cells which further indicating increase in dendritic cell population.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Bright signals with less spill in multicolor flow cytometry.

The Bad

None.

The Bottom Line

Highly recommended.

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