Shivali Rawat

Postdoctoral Researcher

Stanford University

Autophagy Marker Antibody From Abcam
September 22, 2023
MG132, a proteasome inhibitor, is known to cause activation of autophagy. LC3B is cleaved during the activation of autophagy and ... read more
Easy to Use Luciferase Detection Assay Kit From ThermoFisher
September 01, 2023
Firefly luciferase protein is sensitive to proteostasis stress. A double mutant of the protein is conformationally unstable and ... read more
Simple and Cheap Beads for Immunoprecipitation From Santa Cruz
September 01, 2023
I regularly use Protein A/G agarose beads to perform immunoprecipitation experiments followed by Western blotting to detect my ... read more
HSP60 Antibody From Abcam as a Mitochondrial Loading Control
August 24, 2023
HSP60 is a highly abundant and soluble mitochondrial protein. I used it as a loading control for western blots of mitochondrial ... read more
ROS Detection Assay Kit From Abcam
August 21, 2023
In my study, proteostasis stress resulted in the aggregation of mitochondrial proteins inside cells. To investigate the effect on ... read more
VDAC Antibody for Mitochondrial Loading Control From Abcam
August 21, 2023
To study mitochondrial proteins and their activity, I isolated mitochondria from cells. To check the purity of mitochondria and ... read more
Antibody for Complex I Subunit NDUFS1 From Abcam
August 21, 2023
NDUFS1 is one of the subunits of mitochondrial respiratory complex I. We used it as a representative subunit to see distribution ... read more
Must Have Imaging System for Cell Culture Labs From Invitrogen
August 16, 2023
Transient transfections, cell staining, and stable cell line preparation are frequent and common experiments that I perform while ... read more
Best Transfection Reagent From Thermo Fisher
August 16, 2023
I frequently perform cell culture transfections of my protein of interest tagged with fluorescent proteins for either stable cell ... read more
Fluorescent Dye From Thermo Fisher for Mitochondria Membrane Potential
August 16, 2023
TMRM is a cell-permeant, cationic, red fluorescent dye that is readily sequestered by active mitochondria and can be detected by ... read more
Good Beta-Actin Antibody From Abcam
August 16, 2023
I use actin antibody as a loading control for my Western blots. It especially works great for insoluble protein fractions. read more
Good Beta-Tubulin Antibody From Abcam
August 16, 2023
I use beta-tubulin antibody as a loading control for protein gels as it does change under various stress conditions. read more
Good and Cheap Flag Antibody From Proteintech
August 15, 2023
The antibody works well for detecting flag-tagged proteins in Western blot. It can also be used for immunoprecipitation. read more
Good and Cheap MYC Tag Antibody From Proteintech
August 15, 2023
The antibody works great for detecting myc-tagged proteins in Western blot. It gives a good signal even in higher dilutions. read more
Antibody for Complex IV Subunit MT-CO1
August 10, 2023
MT-CO1 is one of the subunits of mitochondrial respiratory complex IV. We used it as a representative subunit to see distribution ... read more

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Reliable RNase A for Evaluating mRNA Protection in Lipid Nanoparticles

Gherardo Baudo

I used Thermo Scientific RNase A to degrade unprotected mRNA during a gel retention assay to evaluate the protective effect of lipid nanoparticles (LNPs). Using... read more

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