Sensitive dsDNA Quantification

October 20, 2026

BlueskyReddit
Stanford University
Department of Pathology
Postdoctoral Researcher

Overall

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Questions/Comments

Company:

Thermo Fisher Scientific

Product Name:

Qubit™ 1X dsDNA High Sensitivity (HS) Assay Kit

Catalog Number:

Q33230

I work with DNA samples for molecular biology and genome-editing experiments. The Qubit 1X dsDNA assay provides a convenient way to quantify DNA accurately, when samples are limited and high sensitivity is required for deep sequencing.

Experimental Design and Results Summary

Application

Fluorometric quantification of dsDNA

Starting Material

Purified PCR products

Tips

Use the supplied standards for calibrating before measurement

Results Summary

Provides accurate measurement of dsDNA as low as 10ng/ul. It is sensitive, selective dsDNA quantification with less interference from RNA, protein, salts, and free nucleotides than other methods

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Summary

The Good

Ready-to-use reagent simplifies the workflow, high sensitivity for low-concentration DNA samples.

The Bad

Requires a compatible Qubit Fluorometer.

The Bottom Line

A convenient, sensitive option for DNA quantification, especially when accurate measurements of low-concentration samples are important.

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