Flag Antibody from Sigma for IF

September 27, 2026

BlueskyReddit
Department of Pathology
Stanford University
Postdoctoral Researcher

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Company:

MilliporeSigma

Product Name:

ANTI-FLAG antibody

Catalog Number:

F7425

Info:Supplier Page

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I study gene signaling pathways and use FLAG-tagged proteins to examine protein expression and localization. This antibody provides strong, specific detection of FLAG-tagged proteins in cells by immunofluorescence.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Transfected mammalian cells

Primary Incubation

1:500, 1 hour, Room temperature

Blocking Agent

Donkey serum and BSA

Secondary Incubation

1:500, 1 hour, Room temperature

Tertiary Incubation

DAPI in mounting media

Detection

Confocal microscopy

Results Summary

I obtained clear FLAG staining, making it useful to identify transfected cells and visualize the localization of my FLAG-tagged proteins.

Additional Notes

Use a secondary antibody control to confirm absence of non-specific staining

Image Gallery

Summary

The Good

Bright, clean staining

The Bad

Expensive

The Bottom Line

A reliable FLAG antibody for IF that gives strong, clear staining of FLAG-tagged proteins.

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